Skip to main content

Application of Tissue Microarray in Esophageal Adenocarcinoma

  • Protocol
  • First Online:
Book cover Esophageal Adenocarcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1756))

Abstract

Tissue microarray technology could allow immunohistochemical staining or in situ hybridization on hundreds of different tissue samples simultaneously. It allows faster analysis and considerably reducing costs incurred in staining. The technique also provides a high-throughput analysis of multiple tissues for the different types of research. In the literature, many researches of esophageal adenocarcinoma use tissue microarray to enhance the output. In this chapter, we have a brief overview of tissue microarray technologies, the advantages and disadvantages of tissue microarray, and related troubleshootings.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Simon R, Mirlacher M, Sauter G (2005) Tissue microarrays. Methods Mol Med 114:257–268

    CAS  PubMed  Google Scholar 

  2. Langer R, Von Rahden BH, Nahrig J, Von Weyhern C, Reiter R, Feith M, Stein HJ, Siewert JR, Höfler H, Sarbia M (2006) Prognostic significance of expression patterns of c-erbB-2, p53, p16INK4A, p27KIP1, cyclin D1 and epidermal growth factor receptor in esophageal adenocarcinoma: a tissue microarray study. J Clin Pathol 59:631–634

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Kuester D, Dar AA, Moskaluk CC, Krueger S, Meyer F, Hartig R, Stolte M, Malfertheiner P, Lippert H, Roessner A, El-Rifai W, Schneider-Stock R (2007) Early involvement of death-associated protein kinase promoter hypermethylation in the carcinogenesis of Barrett’s esophageal adenocarcinoma and its association with clinical progression. Neoplasia 9:236–245

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Boult J, Roberts K, Brookes MJ, Hughes S, Bury JP, Cross SS, Anderson GJ, Spychal R, Iqbal T, Tselepis C (2008) Overexpression of cellular iron import proteins is associated with malignant progression of esophageal adenocarcinoma. Clin Cancer Res 14:379–387

    Article  CAS  PubMed  Google Scholar 

  5. Thompson SK, Sullivan TR, Davies R, Ruszkiewicz AR (2011) Her-2/neu gene amplification in esophageal adenocarcinoma and its influence on survival. Ann Surg Oncol 18:2010–2017

    Article  PubMed  Google Scholar 

  6. Peters JH, Zhou Z (2011) HER2 amplification, overexpression and score criteria in esophageal adenocarcinoma. Mod Pathol 24:899–907

    Article  PubMed  PubMed Central  Google Scholar 

  7. Storr SJ, Pu X, Davis J, Lobo D, Reece-Smith AM, Parsons SL, Madhusudan S, Martin SG (2013) Expression of the calpain system is associated with poor clinical outcome in gastro-esophageal adenocarcinomas. J Gastroenterol 48:1213–1221

    Article  CAS  PubMed  Google Scholar 

  8. Prins MJ, Ruurda JP, Lolkema MP, Sitarz R, Ten Kate FJ, van Hillegersberg R (2015) The role of biological markers of epithelial to mesenchymal transition in esophageal adenocarcinoma, an immunohistochemical study. J Clin Pathol 68:529–535

    Article  CAS  PubMed  Google Scholar 

  9. Stein AV, Dislich B, Blank A, Guldener L, Kröll D, Seiler CA, Langer R (2017) High intratumoural but not peritumoural inflammatory host response is associated with better prognosis in primary resected esophageal adenocarcinomas. Pathology 49:30–37

    Article  PubMed  Google Scholar 

  10. Prins MJ, Verhage RJ, Ruurda JP, ten Kate FJ, van Hillegersberg R (2013) Over-expression of phosphorylated mammalian target of rapamycin is associated with poor survival in esophageal adenocarcinoma: a tissue microarray study. J Clin Pathol 66:224–228

    Article  CAS  PubMed  Google Scholar 

  11. Dibb M, Han N, Choudhury J, Hayes S, Valentine H, West C, Ang YS, Sharrocks AD (2016) The FOXM1-PLK1 axis is commonly upregulated in esophageal adenocarcinoma. Br J Cancer 107:1766–1775

    Article  Google Scholar 

  12. Geppert CI, Rümmele P, Sarbia M, Langer R, Feith M, Morrison L, Pestova E, Schneider-Stock R, Hartmann A, Rau TT (2014) Multi-colour FISH in esophageal adenocarcinoma-predictors of prognosis independent of stage and grade. Br J Cancer 110:2985–2995

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Kononen J, Bubendorf L, Kallioniemi A, Bärlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP (1998) Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 4:844–847

    Article  CAS  PubMed  Google Scholar 

  14. Garcia JF, Camacho FI, Morente M, Fraga M, Montalbán C, Alvaro T, Bellas C, Castaño A, Díez A, Flores T, Martin C, Martinez MA, Mazorra F, Menárguez J, Mestre MJ, Mollejo M, Sáez AI, Sánchez L, Piris MA, Spanish Hodgkin Lymphoma Study Group (2002) Hodgkin’s and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue-microarrays. Blood 12:681–689

    Google Scholar 

  15. Merseburger AS, Kuczyk MA, Serth J, Bokemeyer C, Young DY, Sun L, Connelly RR, McLeod DG, Mostofi FK, Srivastava SK, Stenzl A, Moul JW, Sesterhenn IA (2003) Limitations of tissue microarrays in the evaluation of focal alterations of bcl-2 and p53 in whole mount derived prostate tissues. Oncol Rep 10:223–228

    CAS  PubMed  Google Scholar 

  16. Hedvat CV, Hegde A, Chaganti RS, Chen B, Qin J, Filippa DA, Nimer SD, Teruya-Feldstein J (2002) Application of tissue microarray technology to the study of non-Hodgkin's and Hodgkin’s lymphoma. Hum Pathol 33:968–974

    Article  PubMed  Google Scholar 

  17. Natkunam Y, Warnke RA, Montgomery K, Falini B, van De Rijn M (2001) Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry. Mod Pathol 14:686–694

    Article  CAS  PubMed  Google Scholar 

  18. Rubin MA, Dunn R, Strawderman M, Pienta KJ (2002) Tissue microarray sampling strategy for prostate cancer biomarker analysis. Am J Surg Pathol 26:312–319

    Article  PubMed  Google Scholar 

  19. Hoos A, Urist MJ, Stojadinovic A, Mastorides S, Dudas ME, Leung DH, Kuo D, Brennan MF, Lewis JJ, Cordon-Cardo C (2001) Validation of tissue microarrays for immunohistochemical profiling of cancer specimens using the example of human fibroblastic tumors. Am J Pathol 158:1245–1251

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Engellau J, Akerman M, Anderson H, Domanski HA, Rambech E, Alvegård TA, Nilbert M (2001) Tissue microarray technique in soft tissue sarcoma: immunohistochemical Ki-67 expression in malignant fibrous histiocytoma. Appl Immunohistochem Mol Morphol 9:358–363

    CAS  PubMed  Google Scholar 

  21. Schraml P, Kononen J, Bubendorf L, Moch H, Bissig H, Nocito A, Mihatsch MJ, Kallioniemi OP, Sauter G (1999) Tissue microarrays for gene amplification surveys in many different tumor types. Clin Cancer Res 5:1966–1975

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Alfred K. Lam .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Saremi, N., Lam, A.K. (2018). Application of Tissue Microarray in Esophageal Adenocarcinoma. In: Lam, A. (eds) Esophageal Adenocarcinoma. Methods in Molecular Biology, vol 1756. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7734-5_10

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7734-5_10

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7733-8

  • Online ISBN: 978-1-4939-7734-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics