Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models

  • Minzhong Yu
  • Neal S. Peachey
Part of the Methods in Molecular Biology book series (MIMB, volume 1753)


A monolayer of pigmented epithelial cells, the retinal pigment epithelium (RPE), supports photoreceptor function in many ways. Consistent with these roles, RPE dysfunction underlies a number of hereditary retinal disorders. To monitor RPE function in vivo models for these conditions, we adapted an electroretinographic (ERG) technique based on direct current amplification (DC-ERG). This chapter describes the main features of this approach and its application to mouse models involving the RPE.

Key words

Direct current electroretinography Retinal pigment epithelium C-wave Fast oscillation Light peak Off-response 


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Ophthalmic Research, Cole Eye InstituteCleveland Clinic FoundationClevelandUSA
  2. 2.Department of OphthalmologyCleveland Clinic Lerner College of Medicine of Case Western Reserve UniversityClevelandUSA
  3. 3.Louis Stokes Cleveland VA Medical CenterClevelandUSA

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