Determination of Mitochondrial Oxygen Consumption in the Retina Ex Vivo: Applications for Retinal Disease

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1753)

Abstract

Mitochondrial dysfunction, and consequently altered aerobic energy metabolism, is associated with numerous retinal diseases, including photoreceptor degeneration and diabetic retinopathy. Here, we describe a detailed protocol to directly measure oxygen consumption in the intact retina ex vivo using microplate-based fluorescence technology. We have used this method to assess preferred energy substrate for retinal tissue and suggested its application for investigating mechanisms of retinal disease.

Key words

Aerobic respiration Energy metabolism Photoreceptor Retinal degeneration Mouse models 

Notes

Acknowledgment

The authors are supported by the Intramural Research Program (EY000450 and EY000546) of the National Eye Institute, National Institutes of Health, Bethesda, MD, USA. YKA is supported in part by Department of Science and Technology INSPIRE Faculty award (Govt. of India). We thank Drs. Tiziana Cogliati and Jacob Nellissery for comments on the manuscript and Drs. Shan Chen and Raul Covian for technical advice.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Neurobiology-Neurodegeneration & Repair LaboratoryNational Eye Institute, National Institutes of HealthBethesdaUSA
  2. 2.Department of Cellular and Molecular NeuroscienceNational Brain Research CentreManesarIndia

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