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Microglia Analysis in Retinal Degeneration Mouse Models

  • Katharina Dannhausen
  • Khalid Rashid
  • Thomas Langmann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1753)

Abstract

Microgliosis is a hallmark of degenerative processes in the retina. Reactive microglia migrate to the photoreceptor layer and the subretinal space during outer retinal degeneration. This process creates a toxic milieu where reactive microglia and dying photoreceptors recruit additional reactive phagocytes. This results in the release of a multitude of proinflammatory factors which accelerate photoreceptor demise. In this chapter, we outline in detail how to monitor microgliosis in the Fam161a-deficient mouse model of Retinitis Pigmentosa by performing immunohistochemical stainings of retinal cryosections and flat mounts using the marker Iba1. This protocol will serve as a guideline in evaluating microglia reactivity and localization in various mouse models of retinal degeneration.

Key words

Microglia Retinal degeneration Immunohistochemistry Cryosections Flat mounts Iba1 Fam161a 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Katharina Dannhausen
    • 1
  • Khalid Rashid
    • 1
  • Thomas Langmann
    • 1
  1. 1.Laboratory for Experimental Immunology of the Eye, Department of OphthalmologyUniversity of CologneCologneGermany

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