Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting
Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the isolation of differentiating mouse embryonic cardiac progenitors and cardiomyocytes at embryonic day (E) 9.5 and E13.5, respectively by FACS. Over 50,000 differentiating cardiac progenitors and 200,000 cardiomyocytes can be obtained in a single prep using the methods described.
Key wordsFluorescent-activated cell sorting Cardiac progenitor isolation Cardiomyocyte isolation Mouse embryo
We thank The SickKids-UHN Flow Cytometry Facility for help with FACS, and The Centre for Phenogenomics (TCP) for mouse husbandry. This work was supported by the Heart and Stroke Foundation of Canada (G-17-0018613), the Natural Sciences and Engineering Research Council of Canada (NSERC) (500865), the Canadian Institutes of Health Research (CIHR) (PJT-149046), and Operational Funds from the Hospital for Sick Children to P.D.-O.
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