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Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting

  • Abdalla Ahmed
  • Paul Delgado-Olguin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1752)

Abstract

Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the isolation of differentiating mouse embryonic cardiac progenitors and cardiomyocytes at embryonic day (E) 9.5 and E13.5, respectively by FACS. Over 50,000 differentiating cardiac progenitors and 200,000 cardiomyocytes can be obtained in a single prep using the methods described.

Key words

Fluorescent-activated cell sorting Cardiac progenitor isolation Cardiomyocyte isolation Mouse embryo 

Notes

Acknowledgment

We thank The SickKids-UHN Flow Cytometry Facility for help with FACS, and The Centre for Phenogenomics (TCP) for mouse husbandry. This work was supported by the Heart and Stroke Foundation of Canada (G-17-0018613), the Natural Sciences and Engineering Research Council of Canada (NSERC) (500865), the Canadian Institutes of Health Research (CIHR) (PJT-149046), and Operational Funds from the Hospital for Sick Children to P.D.-O.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Translational MedicineThe Hospital for Sick Children, Peter Gilgan Centre for Research and LearningTorontoCanada
  2. 2.Department of Molecular GeneticsUniversity of TorontoTorontoCanada
  3. 3.Translational MedicineThe Hospital for Sick ChildrenTorontoCanada
  4. 4.Department of Molecular GeneticsUniversity of TorontoTorontoCanada
  5. 5.Heart & Stroke/Richard Lewar Centres of Excellence in Cardiovascular ResearchTorontoCanada

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