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Embryonary Mouse Cardiac Fibroblast Isolation

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Mouse Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1752))

Abstract

Mouse cardiac fibroblasts have been widely used as an in vitro model for studying fundamental biological processes and mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Cardiac FBs are relatively easy to culture in a dish and can be manipulated using molecular and pharmacological tools. Because FBs rapidly decrease cell cycle division and proliferative rate after birth, they are prone to phenotypic changes and senescence in cell culture soon after a few passages. Therefore, primary cultures of differentiated fibroblasts from embryos are more desirable. Below we will describe a method that provides good cell yield and viability of E16 CD-1 mouse embryonic cardiac fibroblasts in primary cultures.

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Author information

Authors and Affiliations

  1. Department of Medicine, School of Medicine, University of California, San Diego, CA, USA

    Alejandra Garate-Carrillo

  2. Escuela de Medicina, Universidad Panamericana, México, Cuidad de México, Mexico

    Israel Ramirez

Authors
  1. Alejandra Garate-Carrillo
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  2. Israel Ramirez
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Corresponding author

Correspondence to Israel Ramirez .

Editor information

Editors and Affiliations

  1. Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada

    Paul Delgado-Olguin

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Garate-Carrillo, A., Ramirez, I. (2018). Embryonary Mouse Cardiac Fibroblast Isolation. In: Delgado-Olguin, P. (eds) Mouse Embryogenesis. Methods in Molecular Biology, vol 1752. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7714-7_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7714-7_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7713-0

  • Online ISBN: 978-1-4939-7714-7

  • eBook Packages: Springer Protocols

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