Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac
Whole mount immunofluorescence is a valuable technique that can be used to visualize vascular networks in early developing embryonic tissues. This technique involves the permeabilization of fixed mouse embryos and yolk sacs, and primary antibody tagging of the endothelial cell marker platelet endothelial cell adhesion molecule 1 (Pecam-1). A secondary antibody tagged with a fluorophore targets the primary antibody, fluorescently labeling endothelial cells and revealing vascular networks.
Key wordsWhole mount immunofluorescence Vascular network Embryo Yolk sac
This work was supported by the Heart and Stroke Foundation of Canada (G-17-0018613), the Natural Sciences and Engineering Research Council of Canada (NSERC) (500865), the Canadian Institutes of Health Research (CIHR) (PJT-149046), and Operational Funds from the Hospital for Sick Children to P.D.-O.
- 6.Ursini-Siegel J, Beauchemin N (eds) (2016) The tumor microenvironment: methods and protocols. Meth Mol Biol 1458. https://doi.org/10.1007/978-1-4939-3801-8_1