Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1752)

Abstract

Whole mount immunofluorescence is a valuable technique that can be used to visualize vascular networks in early developing embryonic tissues. This technique involves the permeabilization of fixed mouse embryos and yolk sacs, and primary antibody tagging of the endothelial cell marker platelet endothelial cell adhesion molecule 1 (Pecam-1). A secondary antibody tagged with a fluorophore targets the primary antibody, fluorescently labeling endothelial cells and revealing vascular networks.

Key words

Whole mount immunofluorescence Vascular network Embryo Yolk sac 

Notes

Acknowledgment

This work was supported by the Heart and Stroke Foundation of Canada (G-17-0018613), the Natural Sciences and Engineering Research Council of Canada (NSERC) (500865), the Canadian Institutes of Health Research (CIHR) (PJT-149046), and Operational Funds from the Hospital for Sick Children to P.D.-O.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Translational MedicineThe Hospital for Sick ChildrenTorontoCanada
  2. 2.Department of Molecular GeneticsUniversity of TorontoTorontoCanada
  3. 3.Genetics & Genome BiologyPeter Gilgan Centre for Research and Learning, The Hospital for Sick ChildrenTorontoCanada
  4. 4.Translational MedicineThe Hospital for Sick ChildrenTorontoCanada
  5. 5.Department of Molecular GeneticsUniversity of TorontoTorontoCanada
  6. 6.Heart & Stroke/Richard Lewar Centres of Excellence in Cardiovascular ResearchTorontoCanada

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