Chromosome Painting of Mouse Chromosomes
Chromosome painting enables the visualization of chromosomes and has been used extensively in cytogenetics. Chromosome paint probes, which consist of a pooled composite of DNA-FISH probes, bind to nonrepetitive sequences for individual chromosomes [1, 2]. Here we describe the process of using chromosome paint to study the organization of chromosomes without fragmenting the nucleus. This method can be used to analyze chromosome position, and identify translocations and ploidy within the nucleus. The preservation of nuclear morphology is crucial in understanding interchromosomal interactions and dynamics in the nucleus during the cell cycle.
Key wordsChromosome paint DNA-FISH Fluorescence in situ hybridization Nuclear organization Nuclear structure Chromosomes Cytogenetics
We would like to thank Sara Venters and all the other members of the Mikawa lab for helpful discussions on technical details of the protocol. We would also like to thank Karen Leung (Barbara Panning lab), Eirene Markenscoff-Papadimitriou (Stavros Lomvardas lab), Kiichiro Tomoda (Shinya Yamanaka lab), and Stephanie Parker (Maximiliano D’Angelo lab) for helpful discussions. This work was supported by NIH grant R37HL078921, R01HL112268, R01HL122375, and R01HL132832 to T.M.
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