Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APC) Populations
Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immunophenotyping, quantification, and isolation of distinct populations, which is critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of adipocyte precursor cells using flow cytometry and FACS, defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the plasticity and the cellular origin of APCs.
Key wordsFlow cytometry Adipocyte precursor cells (APCs) Lineage tracing Cre-recombinase transgenic mouse model Adipose tissue biology
This work was funded by grants from the Natural Sciences and Engineering Research Council (NSERC) of Canada, Pilot and Feasibility Study Grant of Banting & Best Diabetes Centre (BBDC), Centre for Healthy Active Kids (CHAK) Micro-grant and Sickkids Start-up fund to H.-K.S. J.-H.M is supported by the Restracomp fellowship from The Hospital for Sick Children.