Mouse Genotyping

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1752)

Abstract

Genotyping is an invaluable tool for identifying organisms carrying genetic variations including insertions and deletions. This method involves the extraction of DNA from animal tissue samples and subsequent amplification of genomic regions of interest by polymerase chain reaction (PCR). The amplified products are analyzed by agarose gel electrophoresis based on their size.

Key words

Genotype DNA Polymerase chain reaction 

Notes

Acknowledgment

This work was supported by the Heart and Stroke Foundation of Canada (G-17-0018613), the Natural Sciences and Engineering Research Council of Canada (NSERC) (500865), the Canadian Institutes of Health Research (CIHR) (PJT-149046), and Operational Funds from the Hospital for Sick Children to P.D.-O.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Translational MedicineThe Hospital for Sick ChildrenTorontoCanada
  2. 2.Department of Molecular GeneticsUniversity of TorontoTorontoCanada
  3. 3.Heart & Stroke Richard Lewar Centres of Excellence in Cardiovascular ResearchTorontoCanada

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