Abstract
High-resolution mass spectrometry provides the resolution required for direct infusion allowing detection and characterization of a vast array of lipids with a single injection. This chapter presents the methodology utilized for both unbiased and targeted lipidomics of cerebrospinal fluid.
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References
Kantae V, Ogino S, Noga M, Harms AC, van Dongen RM, Onderwater GL, van den Maagdenberg AM, Terwindt GM, van der Stelt M, Ferrari MD, Hankemeier T (2017) Quantitative profiling of endocannabinoids and related N-acylethanolamines in human CSF using nano LC-MS/MS. J Lipid Res 58:615–624
Seyer A, Boudah S, Broudin S, Junot C, Colsch B (2016) Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow. Metabolomics 12:91
Gonzalo H, Brieva L, Tatzber F, Jové M, Cacabelos D, Cassanyé A, Lanau-Angulo L, Boada J, Serrano JC, González C, Hernández L, Peralta S, Pamplona R, Portero-Otin M (2012) Lipidome analysis in multiple sclerosis reveals protein lipoxidative damage as a potential pathogenic mechanism. J Neurochem 123:622–634
Koal T, Klavins K, Seppi D, Kemmler G, Humpel C (2015) Sphingomyelin SM(d18:1/18:0) is significantly enhanced in cerebrospinal fluid samples dichotomized by pathological amyloid-β42, tau, and phospho-tau-181 levels. J Alzheimers Dis 44:1193–1201
Trushina E, Dutta T, Persson XM, Mielke MM, Petersen RC (2013) Identification of altered metabolic pathways in plasma and CSF in mild cognitive impairment and Alzheimer’s disease using metabolomics. PLoS One 8:e63644
Kaddurah-Daouk R, Zhu H, Sharma S, Bogdanov M, Rozen SG, Matson W, Oki NO, Motsinger-Reif AA, Churchill E, Lei Z, Appleby D, Kling MA, Trojanowski JQ, Doraiswamy PM, Arnold SE, Pharmacometabolomics Research Network (2013) Alterations in metabolic pathways and networks in Alzheimer's disease. Transl Psychiatry 3:e244
Ibáñez C, Simó C, Martín-Álvarez PJ, Kivipelto M, Winblad B, Cedazo-Mínguez A, Cifuentes A (2012) Toward a predictive model of Alzheimer’s disease progression using capillary electrophoresis-mass spectrometry metabolomics. Anal Chem 84:8532–8540
Wood PL, Barnette BL, Kaye JA, Quinn JF, Woltjer RL (2015) Non-targeted lipidomics of CSF and frontal cortex gray and white matter in control, mild cognitive impairment, and Alzheimer’s disease subjects. Acta Neuropsychiatrica 27:270–278
Wood PL (2017) Non-targeted lipidomics utilizing constant infusion high resolution ESI mass spectrometry. In Springer Protocols. Meuromethods. Lipidomics 125:13–19 (PL Wood, Ed)
Wood PL (2017) High-resolution mass spectrometry of glycerophospholipid oxidation products. In Springer Protocols Neuromethods. Lipidomics 125:237–241(PL Wood, Ed)
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This work was supported by Lincoln Memorial University.
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Wood, P.L., Woltjer, R.L. (2018). CSF Lipidomics Analysis: High-Resolution Mass Spectrometry Analytical Platform. In: Perneczky, R. (eds) Biomarkers for Alzheimer’s Disease Drug Development. Methods in Molecular Biology, vol 1750. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7704-8_4
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DOI: https://doi.org/10.1007/978-1-4939-7704-8_4
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