Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay

  • Selma Maacha
  • Simon SauleEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1749)


Metastases is largely responsible for the mortality among cancer patients. Metastasis formation is a complex multistep process, which results from the propagation of cancer cells from the primary tumor to distant sites of the body. Research on cancer metastasis aims to understand the mechanisms involved in the spread of cancer cells through the development of in vivo assays that assess cell invasion. Here we describe the use of the chick chorioallantoic membrane to evaluate cancer cell invasiveness in vivo. The chick chorioallantoic membrane assay is based on the detection and quantification of disseminated human tumor cells in the chick embryo femurs by real-time PCR amplification of human Alu sequences.

Key words

Metastasis Migration Invasion Extravasation Chorioallantoic membrane 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of SurgeryVanderbilt University Medical CenterNashvilleUSA
  2. 2.Unité Mixte de Recherche 3347 (UMR), Unité 1021, Centre National de La Recherche Scientifique (CNRS), Institut National de la Santé Et de la Recherche Médicale (INSERM)Institut Curie, PSL Research UniversityOrsayFrance
  3. 3.Unité Mixte de Recherche 3347, Unité 1021, Centre National de La Recherche ScientifiqueUniversité Paris Sud, Université Paris-SaclayOrsayFrance

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