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Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay

  • Selma Maacha
  • Simon SauleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1749)

Abstract

Metastases is largely responsible for the mortality among cancer patients. Metastasis formation is a complex multistep process, which results from the propagation of cancer cells from the primary tumor to distant sites of the body. Research on cancer metastasis aims to understand the mechanisms involved in the spread of cancer cells through the development of in vivo assays that assess cell invasion. Here we describe the use of the chick chorioallantoic membrane to evaluate cancer cell invasiveness in vivo. The chick chorioallantoic membrane assay is based on the detection and quantification of disseminated human tumor cells in the chick embryo femurs by real-time PCR amplification of human Alu sequences.

Key words

Metastasis Migration Invasion Extravasation Chorioallantoic membrane 

References

  1. 1.
    Mehlen P, Puisieux A (2006) Metastasis: a question of life or death. Nat Rev Cancer 6:449–458.  https://doi.org/10.1038/nrc1886 CrossRefPubMedGoogle Scholar
  2. 2.
    Hanahan D, Weinberg RA (2011) Hallmarks of cancer: the next generation. Cell 144:646–674.  https://doi.org/10.1016/j.cell.2011.02.013 CrossRefPubMedGoogle Scholar
  3. 3.
    Maacha S, Anezo O, Foy M et al (2016) Protein tyrosine phosphatase 4A3 (PTP4A3) promotes human uveal melanoma aggressiveness through membrane accumulation of matrix metalloproteinase 14 (MMP14). Invest Ophthalmol Vis Sci 57:1982–1990.  https://doi.org/10.1167/iovs.15-18780 CrossRefPubMedGoogle Scholar
  4. 4.
    Zijlstra A, Mellor R, Panzarella G et al (2002) A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction. Cancer Res 62:7083–7092PubMedGoogle Scholar
  5. 5.
    Palmer TD, Lewis J, Zijlstra A (2011) Quantitative analysis of cancer metastasis using an avian embryo model. J Vis Exp.  https://doi.org/10.3791/2815
  6. 6.
    Kain KH, Miller JWI, Jones-Paris CR et al (2014) The chick embryo as an expanding experimental model for cancer and cardiovascular research. Dev Dyn 243:216–228.  https://doi.org/10.1002/dvdy.24093 CrossRefPubMedGoogle Scholar
  7. 7.
    Kim Y, Williams KC, Gavin CT et al (2016) Quantification of cancer cell extravasation in vivo. Nat Protoc 11:937–948.  https://doi.org/10.1038/nprot.2016.050 CrossRefPubMedGoogle Scholar
  8. 8.
    Lokman NA, Elder ASF, Ricciardelli C, Oehler MK (2012) Chick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian cancer invasion and metastasis. Int J Mol Sci 13:9959–9970.  https://doi.org/10.3390/ijms13089959 CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Xiao X, Zhou X, Ming H et al (2015) Chick Chorioallantoic membrane assay: a 3D animal model for study of human nasopharyngeal carcinoma. PLoS One 10:e0130935.  https://doi.org/10.1371/journal.pone.0130935 CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Laurent C, Valet F, Planque N et al (2011) High PTP4A3 phosphatase expression correlates with metastatic risk in uveal melanoma patients. Cancer Res 71:666–674.  https://doi.org/10.1158/0008-5472.CAN-10-0605 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of SurgeryVanderbilt University Medical CenterNashvilleUSA
  2. 2.Unité Mixte de Recherche 3347 (UMR), Unité 1021, Centre National de La Recherche Scientifique (CNRS), Institut National de la Santé Et de la Recherche Médicale (INSERM)Institut Curie, PSL Research UniversityOrsayFrance
  3. 3.Unité Mixte de Recherche 3347, Unité 1021, Centre National de La Recherche ScientifiqueUniversité Paris Sud, Université Paris-SaclayOrsayFrance

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