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3D Endothelial Cell Migration

  • Kathryn A. Jacobs
  • Julie GavardEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1749)

Abstract

Endothelial cells have the capacity to shift between states of quiescence and angiogenesis. The early stage of angiogenesis, sprouting, occurs with the synchronized activities of tip cells, which lead the migration of the sprout, and stalk cells, which elongate this vessel sprout. Here, we describe a method to study in vitro this early and rapid stage of sprouting angiogenesis.

Key words

Sprouting angiogenesis Endothelial cell VEGF Tumor microenvironment Fibrin matrix Conditioned medium 

Notes

Acknowledgment

The authors are thankful to the present and past members of SOAP laboratory, in particular to Sandy Azzi and Lucas Treps who developed the sprouting assays in the team. Research in SOAP team was funded by: Fondation ARC pour la recherche contre le Cancer, Ligue Nationale contre le Cancer comité Pays-de-la-Loire, comité Maine-et-Loire and comité Vendée, and Connect Talent grant from Région Pays-de-la-Loire and Nantes Métropole. KAJ received a doctoral fellowship from Nantes Métropole.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Team SOAP, CRCINA, INSERM, CNRS, Université de NantesNantesFrance

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