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Cell Migration pp 375-386 | Cite as

Microfluidic Devices for Examining the Physical Limits of Migration in Confined Environments

  • Majid Malboubi
  • Asier Jayo
  • Maddy Parsons
  • Guillaume CharrasEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1749)

Abstract

Cell migration plays a key role in many physiological and pathological conditions during which cells migrate primarily in the 3D environments formed by tissues. Microfluidics enables the design of simple devices that can mimic in a highly controlled manner the geometry and dimensions of the interstices encountered by cells in the body. Here we describe the design, fabrication, and implementation of an array of channels with a range of cross sections to investigate migration of cells and cell clusters through confined spaces. By combining this assay with a motorized microscope stage, image data can be acquired with high throughput to determine the physical limits of migration in confined environments and their biological origin.

Key words

Microfluidics Cell deformation Breast cancer cells Multilayer photolithography 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Majid Malboubi
    • 1
    • 2
  • Asier Jayo
    • 3
  • Maddy Parsons
    • 4
  • Guillaume Charras
    • 2
    • 5
    • 6
    Email author
  1. 1.Department of Engineering ScienceUniversity of OxfordOxfordUK
  2. 2.London Centre for NanotechnologyUniversity College LondonLondonUK
  3. 3.Universidad CEU San PabloMadridSpain
  4. 4.Randall Division of Cell and Molecular BiophysicsKing’s College LondonLondonUK
  5. 5.Institute for the Physics of Living SystemsUniversity College LondonLondonUK
  6. 6.Department of Cell and Developmental BiologyUniversity College LondonLondonUK

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