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Cell Migration pp 341-350 | Cite as

Analysis of Random Migration of Dictyostelium Amoeba in Confined and Unconfined Environments

  • Christof Litschko
  • Julia Damiano-Guercio
  • Stefan Brühmann
  • Jan FaixEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1749)

Abstract

Dictyostelium discoideum has proven to be an excellent model to study amoeboid cell migration. During their life cycle, Dictyostelium cells exhibit distinct modes of motility. Individual growth-phase cells explore new territories by random cell migration using the core cell motility machinery, but they can also hunt bacteria by detection and chemotaxis toward the by-product folate. After depletion of nutrients, the cells initiate a developmental program allowing streaming of the cells into aggregation centers by chemotaxis toward cAMP and by cell-to-cell adhesion. Subsequent development is associated with complex rotational movement of the compacted aggregates to drive cell type specific sorting, which in turn is necessary for terminal culmination and formation of fruiting bodies. Here we describe a protocol for the analyses of cell motility of vegetative Dictyostelium cells in unconfined and mechanically confined settings.

Key words

Cell migration Dictyostelium Confinement Cell tracking Agar overlay Random migration Amoeboid motility 

Notes

Acknowledgments

This work was supported by the Deutsche Forschungsgemeinschaft (DFG grants FA 330/9-1 and FA330/11-1).

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Christof Litschko
    • 1
  • Julia Damiano-Guercio
    • 1
  • Stefan Brühmann
    • 1
  • Jan Faix
    • 1
    Email author
  1. 1.Institute for Biophysical ChemistryHannover Medical SchoolHannoverGermany

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