Cell Migration pp 291-311 | Cite as

Using Single-Protein Tracking to Study Cell Migration

  • Thomas Orré
  • Amine Mehidi
  • Sophie Massou
  • Olivier Rossier
  • Grégory GiannoneEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1749)


To get a complete understanding of cell migration, it is critical to study its orchestration at the molecular level. Since the recent developments in single-molecule imaging, it is now possible to study molecular phenomena at the single-molecule level inside living cells. In this chapter, we describe how such approaches have been and can be used to decipher molecular mechanisms involved in cell migration.

Key words

Cell migration Single-protein tracking Superresolution microscopy Integrin-dependent adhesion Lamellipodium 



Acousto-optic tunable filter


CRISPR associated protein 9


Clustered regularly interspaced short palindromic repeats


Diffusion coefficient


Dulbecco’s modified Eagle medium, high glucose


Ethylenediaminetetraacetic acid


Electron-multiplying charge-coupled device


Fetal bovine serum


Full width at half maximum


Green fluorescent protein


Mouse embryonic fibroblast


Monomeric Eos2


Mean square displacement


Numerical aperture


Photoactivation localization microscopy


Phosphate buffered saline


Scientific complementary metal-oxide semiconductor


Superresolved reconstruction image


Superresolution time-lapse movie


Total internal reflection fluorescence


Universal point accumulation imaging in nanoscale topography


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Thomas Orré
    • 1
    • 2
  • Amine Mehidi
    • 1
    • 2
  • Sophie Massou
    • 1
    • 2
  • Olivier Rossier
    • 1
    • 2
  • Grégory Giannone
    • 1
    • 2
    Email author
  1. 1.Interdisciplinary Institute for Neuroscience, UMR 5297University of BordeauxBordeauxFrance
  2. 2.Interdisciplinary Institute for Neuroscience, UMR 5297CNRSBordeauxFrance

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