Migration of Q Cells in Caenorhabditis elegans
During C. elegans larval development, the Q neuroblasts produce their lineage by three rounds of divisions along with continuous cell migrations. Their neuronal progeny is dispersed from the pharynx to the anus. This in vivo system to study cell migration is appealing for several reasons. The lineage development is stereotyped; functional analysis and genomic screens are rendered easy and powerful thanks to powerful tools; transgenic manipulations and genome engineering are efficient and can be conveniently combined with live-cell imaging. Here we describe a series of protocols in Q cell migration studies, including quantifications of progeny position, genetic screening strategies, preparation of migration mutants or transgenic worms expressing related fluorescent proteins, multipositional time-lapse tracking of Q cell migration using confocal microscopy and image analyses of single cell movements and dynamics.
Key wordsCaenorhabditis elegans Q neuroblast CRISPR-Cas9 Time-lapse imaging
This study was supported by the National Natural Science Foundation of China to G.O. and Y.C. (31671451, 31525015, 31561130153, and 31190063), the Junior Thousand Talents Program of China to G.O., and the National Basic Research Program of China to G.O. (973 Program, 2013CB945600).
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