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Cell Migration pp 135-143 | Cite as

Neuronal Precursor Migration in Ex Vivo Brain Slice Culture

  • Fu-Sheng Chou
  • Pei-Shan WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1749)

Abstract

Neuronal migration during fetal brain development is a well-coordinated process between the migrating neurons and their substrates, the basal processes of the radial glial cells (RGCs). The progeny–progenitor relationship between the migrating neurons and the RGCs in the developing fetal brain may make interpretations of the results difficult, because the variable in question may affect both the RGCs and the migrating neurons in different ways. A transplantation assay combining migrating cells and the scaffolding tissue from two different sources may circumvent this issue. We developed an ex vivo brain slice transplantation assay that allows recording of migrating neurons in real time.

Key words

Neuronal migration Neurosphere culturing Neurosphere labeling Brain slice culture Cerebral cortex development 

Notes

Acknowledgment

This work is funded by Children’s Mercy-Kansas City Children’s Research Institute.

References

  1. 1.
    Paridaen JT, Huttner WB (2014) Neurogenesis during development of the vertebrate central nervous system. EMBO Rep 15:351–364CrossRefPubMedPubMedCentralGoogle Scholar
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    Neuronal Migration Disorders Information Page. National Institute of Neurological Disorders and Stroke. https://www.ninds.nih.gov/Disorders/All-Disorders/Neuronal-Migration-Disorders-Information-Page. Accessed 24 May 2017
  3. 3.
    Wang PS, Chou FS, Ramachandran S et al (2016) Crucial roles of the Arp2/3 complex during mammalian corticogenesis. Development 143:2741–2752CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of PediatricsUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Children’s Mercy-Kansas CityKansas CityUSA

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