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Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age

  • Valeria GriecoEmail author
  • Barbara Banco
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1748)

Abstract

Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue specimens is a widely used laboratory technique that allows the demonstration and the direct microscopical observation of antigens within tissue sections by means of specific antibodies. IHC is one the best tools to define the immunophenotype of a cell during maturation or neoplastic transformation.

To investigate the immunophenotype of postnatal rabbit Sertoli cell, from neonatal to adult age, in this chapter, the immunohistochemical protocol described is one of the most common: the avidin-biotin complex (ABC) method.

Keywords

Formalin-fixed specimens Sertoli cells Antigens Antibodies Chromogen 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Reparto di Anatomia Patologica, DiMeVetUniversità degli Studi di MilanoMilanItaly

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