Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age
- 684 Downloads
Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue specimens is a widely used laboratory technique that allows the demonstration and the direct microscopical observation of antigens within tissue sections by means of specific antibodies. IHC is one the best tools to define the immunophenotype of a cell during maturation or neoplastic transformation.
To investigate the immunophenotype of postnatal rabbit Sertoli cell, from neonatal to adult age, in this chapter, the immunohistochemical protocol described is one of the most common: the avidin-biotin complex (ABC) method.
KeywordsFormalin-fixed specimens Sertoli cells Antigens Antibodies Chromogen
- 13.Hayat MA (2002) Fixation and embedding. In: Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy. Kluwer Academic, New York, pp 71–93Google Scholar