Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age

  • Valeria GriecoEmail author
  • Barbara Banco
Part of the Methods in Molecular Biology book series (MIMB, volume 1748)


Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue specimens is a widely used laboratory technique that allows the demonstration and the direct microscopical observation of antigens within tissue sections by means of specific antibodies. IHC is one the best tools to define the immunophenotype of a cell during maturation or neoplastic transformation.

To investigate the immunophenotype of postnatal rabbit Sertoli cell, from neonatal to adult age, in this chapter, the immunohistochemical protocol described is one of the most common: the avidin-biotin complex (ABC) method.


Formalin-fixed specimens Sertoli cells Antigens Antibodies Chromogen 


  1. 1.
    Griswold MD (1995) Interactions between germ cells and Sertoli cells in the testis. Biol Reprod 52:211–216CrossRefPubMedGoogle Scholar
  2. 2.
    Sharpe RM, McKinnell C, Kivlin C, Fisher JS (2003) Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood. Reproduction 125:769–784CrossRefPubMedGoogle Scholar
  3. 3.
    Banco B, Grilli G, Giudice C, Marques AT, Cotti Cometti S, Visigalli G, Grieco V (2016) Immunophenotyping of rabbit testicular germ and Sertoli cells across maturational stages. J Histochem Cytochem 64:715–726CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Williams J, Gladen BC, Turner TW, Schrader SM, Chapin RE (1991) The effects of ethylene dibromide on semen quality and fertility in the rabbit: evaluation of a model for human seminal characteristics. Fundam Appl Toxicol 16:687–700CrossRefPubMedGoogle Scholar
  5. 5.
    Attardi BJ, Engbring JA, Gropp D, Hild SA (2011) Development of dimethandrolone 17beta-undecanoate (DMAU) as an oral male hormonal contraceptive: induction of infertility and recovery of fertility in adult male rabbits. J Androl 32:530–540CrossRefPubMedGoogle Scholar
  6. 6.
    Brown PJ, Stafford RA (1989) A testicular seminoma in a rabbit. J Comp Pathol 100:353–355CrossRefPubMedGoogle Scholar
  7. 7.
    Veeramachaneni DN, Vandewoude S (1999) Interstitial cell tumour and germ cell tumour with carcinoma in situ in rabbit testes. Int J Androl 22:97–101CrossRefPubMedGoogle Scholar
  8. 8.
    Roccabianca P, Ghisleni G, Scanziani E (1999) Simultaneous seminoma and interstitial cell tumour in a rabbit with a previous cutaneous basal cell tumour. J Comp Pathol 121:95–99CrossRefPubMedGoogle Scholar
  9. 9.
    Banco B, Stefanello D, Giudice C, D'Acierno M, Di Giancamillo M, Grieco V (2012) Metastasizing testicular seminoma in a pet rabbit. J Vet Diagn Investig 24:608–611CrossRefGoogle Scholar
  10. 10.
    Banco B, Ferreira da Silva J, Cotti Cometti S, Stefanello D, Grieco V (2017) Immunohistochemical expression of placental alkaline phosphatase in five cases of seminoma in rabbits. J Comp Pathol 156:366–370CrossRefPubMedGoogle Scholar
  11. 11.
    Ramos-Vara JA, Miller MA (2014) When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry--the red, brown, and blue technique. Vet Pathol 51:42–87CrossRefPubMedGoogle Scholar
  12. 12.
    Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580CrossRefPubMedGoogle Scholar
  13. 13.
    Hayat MA (2002) Fixation and embedding. In: Microscopy, immunohistochemistry, and antigen retrieval methods for light and electron microscopy. Kluwer Academic, New York, pp 71–93Google Scholar
  14. 14.
    Rogatsch H, Jezek D, Hittmair A, Mikuz G, Feichtinger H (1996) Expression of vimentin, cytokeratin, and desmin in Sertoli cells of human fetal, cryptorchid, and tumour-adjacent testicular tissue. Virchows Arch 427:497–502CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Reparto di Anatomia Patologica, DiMeVetUniversità degli Studi di MilanoMilanItaly

Personalised recommendations