Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing
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Tissue cryopreservation uses very low temperatures to preserve structurally intact living cells in their natural microenvironment. Cell survival is strongly influenced by the biophysical effects of ice during both the freezing and the subsequent thawing. These effects can be controlled by optimizing the fragment size, type of cryoprotectant, and cooling rate. The challenge is to determine cryopreservation parameters that suit all cell types present in the tissue. Here we describe a quick and convenient protocol for the cryopreservation of testicular tissue using an isopropyl-insulated freezing device, which was validated in both a mouse and a human model.
KeywordsMouse Human Testicular tissue Cryopreservation Isopropyl Slow freezing
This work was supported by a PhD grant from the Agency for Innovation by Science and Technology and research grants from the European Commission Research and Innovation Marie Sklodowska Actions (MSCA)s Seventh Framework program (FP7-People-2013-ITN-603568), the Flemish League against Cancer-Public Utility Foundation, the Scientific Research Foundation Flanders (FWO), University Hospital Brussels (fund Willy Gepts), and the Vrije Universiteit Brussel (Methusalem grant). Y.B. and D.V.S. are postdoctoral fellows of the FWO.
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