Assessing Sertoli Cell Metabolic Activity
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Nuclear magnetic resonance (NMR)-based metabolomics is widely used in the research of metabolic conditions of complex biological systems under various conditions, and its use has been found in the field of male fertility. Here we describe the implementation of total and targeted NMR-based metabolomics in the research on Sertoli cell metabolism. Main principles and techniques of cell medium, cellular extracts, and intact cells are explained, as well as some classical experiments that can give complementary information on the Sertoli cell metabolism.
KeywordsSertoli Fertility Metabolism NMR Intracellular Extracellular Metabolomics Glucose uptake Lipids
This work was supported by the Portuguese Foundation for Science and Technology (FCT) grant PTDC/BIM-MET/4712/2014 and by the Agencia Nacional de Promoción Cientíca y Tecnológica grants PICT 2014/945 and PICT2015/228 and by the Consejo Nacional de Investigaciones Cientícas y Técnicas (CONICET) grant PIP 2015/127.
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