Establishment of Primary Culture of Sertoli Cells
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The successful isolation and culture of Sertoli cells depend on a series of delicate processes of mechanical isolation and enzymatic digestion of the testicular tissue, taking advantage of an array of enzymes (such as DNAse, collagenase, and pancreatin) in order to digest the extracellular matrix components. The complexity of these processes may present some differences depending on the origin of the testicular sample (whole tissue or biopsy) and of the species in question. Rat and mouse Sertoli cells are obtained by a similar protocol, whereas bovine and human Sertoli cells require a more extensive mechanical and enzymatic processing.
KeywordsEnzymatic digestion Mechanical dispersion Primary cell culture Sertoli cells
This work was supported by the “Fundação para a Ciência e a Tecnologia”–FCT (PTDC/BBB-BQB/1368/2014), UMIB (PEst-OE/SAU/UI0215/2014). R.L. Bernardino was also financed by (SFRH/BD/103105/2014).
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