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Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins

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Nitric Oxide

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1747))

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Abstract

Protein S-nitrosylation, the NO-dependent regulatory mechanism, is a posttranslational modification of reactive cysteine thiols to form S-nitrosothiols. The biotin-switch technique (BST) has become a mainstay method for detection of S-nitrosylated proteins in biological samples. On the basis of BST, we describe a surface plasmon resonance (SPR) spectroscopy method for detecting S-nitrosylated proteins. This method can be applied for the indirect determination of S-nitrosylated proteins in biological samples.

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Acknowledgment

This work has been funded by the National Natural Science Foundation of China (Nos. 31370686, 31470686) and Natural Science Foundation of Shandong Province (ZR2013CQ014).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian, China

    Lili Zhang, Pengpeng Shang, Changbao Chen, Jie Zhou & Shuhua Zhu

Authors
  1. Lili Zhang
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  2. Pengpeng Shang
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  3. Changbao Chen
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  4. Jie Zhou
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  5. Shuhua Zhu
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Corresponding author

Correspondence to Shuhua Zhu .

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Editors and Affiliations

  1. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Alexander Mengel

  2. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Christian Lindermayr

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Zhang, L., Shang, P., Chen, C., Zhou, J., Zhu, S. (2018). Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins. In: Mengel, A., Lindermayr, C. (eds) Nitric Oxide. Methods in Molecular Biology, vol 1747. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7695-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7695-9_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7694-2

  • Online ISBN: 978-1-4939-7695-9

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