Quantum Cascade Lasers-Based Detection of Nitric Oxide
Despite the established importance of nitric oxide (NO) in many physiological and molecular processes in plants, most methods for quantifying NO are open to criticism This reflects the differing methods either lacking specificity or sensitivity, or even from an undue dependence of results on experimental conditions (i.e., chemical concentrations, pH, etc.). In this chapter we describe a protocol to measure gaseous NO produced by a biological sample using quantum cascade laser (QCL)-based spectroscopy. This technique is based on absorption of the laser light by the NO molecules which have been passed from a biological sample into an optical s cell that is equipped with two mirrors placed at both ends. This design greatly increases the interaction path length with the NO molecules due to multiple reflections of the light coupled inside the cell. Thus, the method is able to provide online, in planta measurements of the dynamics of NO production, being highly selective and sensitive (down to ppbv levels;1 ppbv = part per billion by volume mixing ratio = 1:10−9).
Key wordsNitric oxide Quantum cascade laser Laser based infrared spectroscopy Multipass cell Drought stress
This work was supported by the Spanish Ministry of Economy and Competitiveness [AGL2016-78965-R], and the European Regional Development Funds, province of Gelderland [2009-010034]. The work of LM was supported by the BBSRC LINK Grant (BB/I016937/1).
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