A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems: Bell Pepper Fruits as a Model

  • José M. PalmaEmail author
  • Carmelo Ruiz
  • Francisco J. Corpas
Part of the Methods in Molecular Biology book series (MIMB, volume 1747)


Nitric oxide (NO) is involved many physiological plant processes, including germination, growth and development of roots, flower setting and development, senescence, and fruit ripening. In the latter physiological process, NO has been reported to play an opposite role to ethylene. Thus, treatment of fruits with NO may lead to delay ripening independently of whether they are climacteric or nonclimacteric. In many cases different methods have been reported to apply NO to plant systems involving sodium nitroprusside, NONOates, DETANO, or GSNO to investigate physiological and molecular consequences. In this chapter a method to treat plant materials with NO is provided using bell pepper fruits as a model. This method is cheap, free of side effects, and easy to apply since it only requires common chemicals and tools available in any biology laboratory.

Key words

Nitric oxide gas Pepper fruits Ripening Nitric oxide fumigation 



This work was financed by the Ministry of Economy and Competitiveness (Grant AGL2015-65104-P).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • José M. Palma
    • 1
    Email author
  • Carmelo Ruiz
    • 1
  • Francisco J. Corpas
    • 1
  1. 1.Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of PlantsEstación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasGranadaSpain

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