Abstract
Approaches based on next-generation sequencing (NGS) coupled with bioinformatics tools have been developed for detecting viruses and viroids infecting herbaceous and woody plants. Here we describe a protocol to extract nucleic acids from citrus bark and enrich them in double-stranded RNAs. These preparations can be efficiently used for generating cDNA libraries that, after pair-end sequencing and bioinformatics analyses, allow efficient identification of the viroids infecting the source plant.
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References
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Acknowledgments
This work was partially supported by the project INNOCI—DGR n. 903 del 15/05/2012—Linee guida per la ricerca e la sperimentazione in agricoltura—Regione Puglia.
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Navarro, B., Di Serio, F. (2018). Double-Stranded RNA-Enriched Preparations to Identify Viroids by Next-Generation Sequencing. In: Pantaleo, V., Chiumenti, M. (eds) Viral Metagenomics. Methods in Molecular Biology, vol 1746. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7683-6_3
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DOI: https://doi.org/10.1007/978-1-4939-7683-6_3
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