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Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level

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Cellular Heterogeneity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1745))

Abstract

In this paper, we review some of the recent advances in cellular heterogeneity and single-cell analysis methods. In modern research of cellular heterogeneity, there are four major approaches: analysis of pooled samples, single-cell analysis, high-throughput single-cell analysis, and lately integrated analysis of cellular population at a single-cell level. Recently developed high-throughput single-cell genetic analysis methods such as RNA-Seq require purification step and destruction of an analyzed cell often are providing a snapshot of the investigated cell without spatiotemporal context. Correlative analysis of multiparameter morphological, functional, and molecular information is important for differentiation of more uniform groups in the spectrum of different cell types. Simplified distributions (histograms and 2D plots) can underrepresent biologically significant subpopulations. Future directions may include the development of nondestructive methods for dissecting molecular events in intact cells, simultaneous correlative cellular analysis of phenotypic and molecular features by hybrid technologies such as imaging flow cytometry, and further progress in supervised and non-supervised statistical analysis algorithms.

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Acknowledgments

Authors are grateful for grant support from Swiss IBD Cohort to N.S.B., Ministry of Science, Kazakhstan to N.S.B. and I.A.V., and RFBR to I.A.V.

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Authors and Affiliations

  1. PCMM-Harvard Medical School, Boston Children’s Hospital, Boston, MA, USA

    Natasha S. Barteneva

  2. Department of Biology, School of Sciences and Technology, Nazarbayev University, Astana, Kazakhstan

    Natasha S. Barteneva & Ivan A. Vorobjev

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  1. Natasha S. Barteneva
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  2. Ivan A. Vorobjev
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Correspondence to Natasha S. Barteneva .

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Editors and Affiliations

  1. PCMM-Harvard Medical School, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. Department of Biology, School of Sciences and Technology, Nazarbayev University, Astana, Kazakhstan

    Ivan A. Vorobjev

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Barteneva, N.S., Vorobjev, I.A. (2018). Heterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level. In: Barteneva, N., Vorobjev, I. (eds) Cellular Heterogeneity. Methods in Molecular Biology, vol 1745. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7680-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7680-5_1

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