Abstract
As the last stage of plant development, senescence can be regulated by a large number of signals such as aging, reproductive growth, nutrient availability, and stresses. Various plant hormones have been shown to be involved in regulating plant senescence. For example, ethylene, abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and strigolactones (SLs) promote senescence, whereas cytokinins (CKs) inhibit senescence. Different hormones regulate senescence via distinct pathways, while cross talks between signaling pathways exist. In senescence-related studies, treating plants with various hormones to alter senescence is a common practice. In this chapter, we summarize experimental procedures of treating detached Arabidopsis leaves with a number of senescence-regulating hormones including ABA, SLs, MeJA, SA peptide hormones.
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References
Avice JC, Etienne P (2014) Leaf senescence and nitrogen remobilization efficiency in oilseed rape (Brassica napus L.) J Exp Bot 65:3813–3824
Sarwat M, Naqvi AR, Ahmad P et al (2013) Phytohormones and microRNAs as sensors and regulators of leaf senescence: assigning macro roles to small molecules. Biotechnol Adv 31:1153–1171
Khan M, Rozhon W, Poppenberger B (2014) The role of hormones in the aging of plants—a mini-review. Gerontology 60:49–55
Arrom L, Munne-Bosch S (2012) Hormonal regulation of leaf senescence in Lilium. J Plant Physiol 169:1542–1550
Finkelstein R (2013) Abscisic Acid synthesis and response. Arabidopsis Book 11:e0166
Zeevaart JAD, Creelman RA (2003) Metabolism and physiology of abscisic acid. Ann Rev Plant Physiol Plant Mol Biol 39:439–473
Lee IC, Hong SW, Whang SS et al (2011) Age-dependent action of an ABA-inducible receptor kinase, RPK1, as a positive regulator of senescence in Arabidopsis leaves. Plant Cell Physiol 52:651–662
Song Y, Xiang F, Zhang G et al (2016) Abscisic acid as an internal integrator of multiple physiological processes modulates leaf senescence onset in Arabidopsis thaliana. Front Plant Sci 7:181
Mou W, Li D, Luo Z et al (2015) Transcriptomic analysis reveals possible influences of ABA on secondary metabolism of pigments, flavonoids and antioxidants in tomato fruit during ripening. PLoS One 10:e0129598
Zhang K, Gan SS (2012) An abscisic acid-AtNAP transcription factor-SAG113 protein phosphatase 2C regulatory chain for controlling dehydration in senescing Arabidopsis leaves. Plant Physiol 158:961–969
Kim J, Chang C, Tucker ML (2015) To grow old: regulatory role of ethylene and jasmonic acid in senescence. Front Plant Sci 6:20
Balbi V, Devoto A (2008) Jasmonate signalling network in Arabidopsis thaliana: crucial regulatory nodes and new physiological scenarios. New Phytol 177:301–318
Schommer C, Palatnik JF, Aggarwal P et al (2008) Control of jasmonate biosynthesis and senescence by miR319 targets. PLoS Biol 6:e230
Seltmann MA, Hussels W, Berger S (2010) Jasmonates during senescence: signals or products of metabolism? Plant Signal Behav 5:1493–1496
Abreu ME, Munne-Bosch S (2009) Salicylic acid deficiency in NahG transgenic lines and sid2 mutants increases seed yield in the annual plant Arabidopsis thaliana. J Exp Bot 60:1261–1271
Morris K, Mackerness SA, Page T et al (2000) Salicylic acid has a role in regulating gene expression during leaf senescence. Plant J 23:677–685
Besseau S, Li J, Palva ET (2012) WRKY54 and WRKY70 co-operate as negative regulators of leaf senescence in Arabidopsis thaliana. J Exp Bot 63:2667–2679
Ueda H, Kusaba M (2015) Strigolactone regulates leaf senescence in concert with ethylene in Arabidopsis. Plant Physiol 169:138–147
Yamada Y, Umehara M (2015) Possible roles of strigolactones during leaf senescence. Plants (Basel) 4:664–677
Kusaba M, Tanaka A, Tanaka R (2013) Stay-green plants: what do they tell us about the molecular mechanism of leaf senescence. Photosynth Res 117:221–234
Chilley P (2003) Polypeptide hormones: signaling molecules in plants. Vitam Horm 66:317–344
Lease KA, Walker JC (2006) The Arabidopsis unannotated secreted peptide database, a resource for plant peptidomics. Plant Physiol 142:831–838
Czyzewicz N, Yue K, Beeckman T et al (2013) Message in a bottle: small signalling peptide outputs during growth and development. J Exp Bot 64:5281–5296
Kong J, Dong Y, Xu L et al (2014) Effects of foliar application of salicylic acid and nitric oxide in alleviating iron deficiency induced chlorosis of Arachis hypogaea L. Bot Stud 55:1–12
Acknowledgments
This work was supported by the National Natural Science Foundation of China (31600991) (to Z.Z.), the Science Foundation for Young Scholars of the Tobacco Research Institute, the Chinese Academy of Agricultural Sciences (2015B02) (to Z.Z.), and the Agricultural Science and Technology Innovation Program (ASTIP-TRIC02).
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Zhang, Z., Guo, Y. (2018). Hormone Treatments in Studying Leaf Senescence. In: Guo, Y. (eds) Plant Senescence. Methods in Molecular Biology, vol 1744. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7672-0_11
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DOI: https://doi.org/10.1007/978-1-4939-7672-0_11
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