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Hormone Treatments in Studying Leaf Senescence

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1744)

Abstract

As the last stage of plant development, senescence can be regulated by a large number of signals such as aging, reproductive growth, nutrient availability, and stresses. Various plant hormones have been shown to be involved in regulating plant senescence. For example, ethylene, abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and strigolactones (SLs) promote senescence, whereas cytokinins (CKs) inhibit senescence. Different hormones regulate senescence via distinct pathways, while cross talks between signaling pathways exist. In senescence-related studies, treating plants with various hormones to alter senescence is a common practice. In this chapter, we summarize experimental procedures of treating detached Arabidopsis leaves with a number of senescence-regulating hormones including ABA, SLs, MeJA, SA peptide hormones.

Key words

Arabidopsis Phytohormones Leaf senescence Gene expression 
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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31600991) (to Z.Z.), the Science Foundation for Young Scholars of the Tobacco Research Institute, the Chinese Academy of Agricultural Sciences (2015B02) (to Z.Z.), and the Agricultural Science and Technology Innovation Program (ASTIP-TRIC02).

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© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Tobacco Research InstituteChinese Academy of Agricultural SciencesQingdaoChina

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