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Analysis of Mitochondrial Markers of Programmed Cell Death

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Plant Programmed Cell Death

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1743))

Abstract

Mitochondria play a crucial role in programmed cell death (PCD) in plants. In most cases of mitochondria-dependent PCD, cytochrome c (Cyt c) released from mitochondria due to the opening of mitochondrial permeability transition pore (MPTP) and the activation of caspase-like proteases. Here we describe the analytic methods of mitochondrial markers of PCD including mitochondria isolation, mitochondrial membrane permeability, mitochondrial inner membrane potential, Cytc release, ATP, and mitochondrial reactive oxygen species (ROS).

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References

  1. Chibucos MC, Collmer CW, Torto-Alalibo T, Gwinn-Giglio M, Lindeberg M, Li D, Tyler BM (2009) Programmed cell death in host-symbiont associations, viewed through the gene ontology. BMC Microbiol 9:S5

    Article  PubMed  PubMed Central  Google Scholar 

  2. Daneva A, Gao Z, van Durme M, Nowack MK (2016) Functions and regulation of programmed cell death in plant development. Annu Rev Cell Dev Biol 32:441–468

    Article  CAS  PubMed  Google Scholar 

  3. Ma W, Xu W, Xu H, Chen Y, He Z, Ma M (2010) Nitric oxide modulates cadmium influx during cadmium-induced programmed cell death in tobacco BY-2 cells. Planta 232:325–335

    Article  CAS  PubMed  Google Scholar 

  4. Zhang Y, Zhang G, Liu P, Song J, Xu G, Cai M (2011) Morphological and physiological responses of root tip cells to Fe2+ toxicity in rice. Acta Physiol Plant 33:683–689

    Article  CAS  Google Scholar 

  5. Li F, Qi J, Zhang G, Lin L, Fang P, Tao A, Xu J (2013) Effect of cadmium stress on the growth, antioxidative enzymes and lipid peroxidation in two kenaf(Hibiscus cannabinus L.) plant seedlings. J Integr Agric 12:610–620

    Article  Google Scholar 

  6. Panda SK, Yamamoto Y, Kondo H, Matsumoto H (2008) Mitochondrial alterations related to programmed cell death in tobacco cells under aluminium stress. C R Biol 331:597–610

    Article  CAS  PubMed  Google Scholar 

  7. Kroemer G, Galluzzi L, Brenner C (2007) Mitochondrial membrane permeablization in cell death. Physiol Rev 87:99–163

    Article  CAS  PubMed  Google Scholar 

  8. Zhan J, Li W, He H, Li C, He LF (2013) Mitochondrial alterations during Al-induced PCD in peanut root tips. Plant Physiol Biochem 75:105–113

    Article  PubMed  Google Scholar 

  9. Huang W, Yang X, Yao S, Lwinoo T, He H, Wang A, Li C, He L (2014) Reactive oxygen species burst induced by aluminum stress triggers mitochondria-dependent programmed cell death in peanut root tip cells. Plant Physiol Biochem 82:76–84

    Article  CAS  PubMed  Google Scholar 

  10. Yu H, Li J (2015) Plant and animal organelles in cell death. J Org Chem 6:13852–13853

    Google Scholar 

  11. Kim R, Emi M, Tanabe K (2006) Role of mitochondria as the gardens of cell death. Cancer Chemother Pharmacol 57:545–553

    Article  CAS  PubMed  Google Scholar 

  12. Bradford MM (1976) A rapid method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  13. Pastorino JG, Tafani M, Rothman RJ, Marcineviciute A, Hoek JB, Farber JL (1999) Functional consequences of the sustained or transient activation by Bax of the mitochondrial permeability transition pore. J Biol Chem 274:31734–31739

    Article  CAS  PubMed  Google Scholar 

  14. Strehler BL (1963) Adenosine-5-triphosphate and creatine phosphate: determination with luciferase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 559–572

    Google Scholar 

  15. Luo G, Wang A (1999) The detection and scavenging of oxygen free radical in plants. In: Shanghai institute of plant physiology of Chinese academy of sciences, Shanghai society for plant physiology(eds) (ed) Modern plant physiology experiment guide. Academic Press, Beijing, pp 308–309

    Google Scholar 

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Acknowledgment

This work is supported by grants from the National Natural Science Foundation of China (No. 31660350, 31660352, 31560346, and 31260296).

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Authors and Affiliations

  1. College of Agronomy, Guangxi University, Nanning, Guangxi, People’s Republic of China

    Dong Xiao, Huyi He, Wenjing Huang, Thet Lwin Oo, Aiqin Wang & Long-Fei He

Authors
  1. Dong Xiao
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  2. Huyi He
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  3. Wenjing Huang
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  4. Thet Lwin Oo
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  5. Aiqin Wang
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  6. Long-Fei He
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Corresponding author

Correspondence to Long-Fei He .

Editor information

Editors and Affiliations

  1. Food Sciences and Human Nutrition Unit, Università Campus Bio-Medico di Roma, Roma, Italy

    Laura De Gara

  2. Food Sciences and Human Nutrition Unit, Università Campus Bio-Medico di Roma, Roma, Italy

    Vittoria Locato

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Xiao, D., He, H., Huang, W., Oo, T.L., Wang, A., He, LF. (2018). Analysis of Mitochondrial Markers of Programmed Cell Death. In: De Gara, L., Locato, V. (eds) Plant Programmed Cell Death. Methods in Molecular Biology, vol 1743. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7668-3_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7668-3_6

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7667-6

  • Online ISBN: 978-1-4939-7668-3

  • eBook Packages: Springer Protocols

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