Hypoxia pp 139-153 | Cite as

Testing Acute Oxygen Sensing in Genetically Modified Mice: Plethysmography and Amperometry

  • Patricia Ortega-Sáenz
  • Candela Caballero
  • Lin Gao
  • José López-Barneo
Part of the Methods in Molecular Biology book series (MIMB, volume 1742)


Monitoring responsiveness to acute hypoxia of whole animals and single cells is essential to investigate the nature of the mechanisms underlying oxygen (O2) sensing. Here we describe the protocols followed in our laboratory to evaluate the ventilatory response to hypoxia in normal and genetically modified animals. We also describe the amperometric technique used to monitor single-cell catecholamine release from chemoreceptor cells in carotid body and adrenal medulla slices.

Key words

Acute oxygen sensing Hypoxia Hyperventilation Plethysmography Single-cell secretion Amperometry Genetically modified mice 



This work was supported by the Botín Foundation, the Spanish Ministry of Economy and Innovation (SAF2012-39343, SAF2016-74990-R), and the European Research Council (ERC Advanced Grant PRJ201502629).


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© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de SevillaSevilleSpain
  2. 2.Departamento de Fisiología Médica y Biofísica, Facultad de MedicinaUniversidad de SevillaSevilleSpain
  3. 3.Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED)MadridSpain
  4. 4.Division of Pulmonary and Critical Care Medicine, Department of MedicineJohns Hopkins University School of MedicineBaltimoreUSA

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