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Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles

  • Julia Small
  • Sabrina Roy
  • Roger Alexander
  • Leonora Balaj
Part of the Methods in Molecular Biology book series (MIMB, volume 1740)

Abstract

Understanding the role of extracellular RNA (exRNA) has emerged as an exciting avenue for biomarker, therapeutic, as well as basic cell–cell communication applications and discoveries. Multiple protocols, kits, and procedures have been developed in the last decade to allow fractionation as well as isolation of subpopulations of macromolecules of interest found in biofluids. Here, we introduce the protocols decision tree developed by the Extracellular RNA Communication Consortium and available on their website (exRNA portal), and compare all methods currently available to the exRNA field and report pros and cons for each platform.

Keywords

Extracellular RNA Exosomes Biomarkers Ribonuclear particles Kits Protocols 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Julia Small
    • 1
  • Sabrina Roy
    • 3
    • 4
  • Roger Alexander
    • 2
  • Leonora Balaj
    • 1
  1. 1.Department of NeurosurgeryMassachusetts General Hospital and Harvard Medical SchoolBostonUSA
  2. 2.Pacific Northwest Diabetes Research InstituteSeattleUSA
  3. 3.Department of NeurosurgeryMassachusetts General HospitalBostonUSA
  4. 4.Human Biology DepartmentUniversity of TorontoCanada

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