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Schwann Cells pp 137-148 | Cite as

Derivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats

  • Y. P. Tsui
  • Graham K. Shea
  • Y. S. Chan
  • Daisy K. Y. ShumEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1739)

Abstract

Our goal is to derive phenotypically stable Schwann cells from bone marrow stromal cells (BMSCs) for use in transplantation studies of central/peripheral nerve injuries. With the adult rat as model, here we describe steps that foster (1) expansion of the BMSC subpopulation of neural progenitors as neurosphere cells, (2) differentiation of the progenitors into Schwann cell-like cells in adherent culture supplemented with soluble factors, and (3) cell-intrinsic switch of Schwann cell-like cells to the Schwann cell fate following co-culture with sensory neurons purified from dorsal root ganglia. The derived Schwann cells retain marker expression despite withdrawal of supplements and neuronal cues, survive passaging and cryopreservation, and, importantly, show functional capacity for myelination.

Key words

BMSC-derived Schwann cells Nerve regeneration Bone marrow stromal cells Myelination Cell-based therapy 

Notes

Acknowledgment

This work was supported by the Strategic Research Theme of The University of Hong Kong, Croucher Foundation MBBS/PhD Scholarship to GKHS, and the Hong Kong Research Grants Council GRF 777810 to D.K.Y.S.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Y. P. Tsui
    • 1
  • Graham K. Shea
    • 2
  • Y. S. Chan
    • 1
  • Daisy K. Y. Shum
    • 1
    Email author
  1. 1.School of Biomedical Sciences, LKS Faculty of MedicineThe University of Hong KongHong KongChina
  2. 2.Department of Orthopaedics and Traumatology, LKS Faculty of MedicineThe University of Hong KongHong KongChina

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