Advertisement

Schwann Cells pp 439-453 | Cite as

Schwann Cell Transplantation Methods Using Biomaterials

  • Christine D. Plant
  • Giles W. PlantEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1739)

Abstract

Biomaterials can be utilized to assist in the transplantation of Schwann cells to the central and peripheral nervous system. The biomaterials can be natural or man-made, and can have preformed shapes or injectable formats. Biomaterials can play multiple roles in cellular transplantation; for example, they can assist with cellular integration and protect Schwann cells from cell death initiated by the lack of a substrate, an occurrence known as “anoikis.” In addition, biomaterials can be engineered to increase cell proliferation and differentiation by the addition of ligands bound to the substrate. Here, we describe the incorporation of Schwann cells to both man-made and natural matrices for in vitro and in vivo measures relevant to Schwann cell transplantation strategies.

Key words

Schwann cells Biomaterials Matrigel Laminin Collagen Fibrinogen 

References

  1. 1.
    Schense JC, Bloch J, Aebischer P, Hubbell JA (2000) Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension. Nat Biotechnol 18(4):415–419.  https://doi.org/10.1038/74473 CrossRefPubMedGoogle Scholar
  2. 2.
    Oudega M, Xu XM (2006) Schwann cell transplantation for repair of the adult spinal cord. J Neurotrauma 23(3-4):453–467.  https://doi.org/10.1089/neu.2006.23.453 CrossRefPubMedGoogle Scholar
  3. 3.
    Chen A, Xu XM, Kleitman N, Bunge MB (1996) Methylprednisolone administration improves axonal regeneration into Schwann cell grafts in transected adult rat thoracic spinal cord. Exp Neurol 138(2):261–276.  https://doi.org/10.1006/exnr.1996.0065 CrossRefPubMedGoogle Scholar
  4. 4.
    Oudega M, Xu XM, Guenard V, Kleitman N, Bunge MB (1997) A combination of insulin-like growth factor-I and platelet-derived growth factor enhances myelination but diminishes axonal regeneration into Schwann cell grafts in the adult rat spinal cord. Glia 19(3):247–258CrossRefPubMedGoogle Scholar
  5. 5.
    Plant GW, Bates ML, Bunge MB (2001) Inhibitory proteoglycan immunoreactivity is higher at the caudal than the rostral Schwann cell graft-transected spinal cord interface. Mol Cell Neurosci 17(3):471–487.  https://doi.org/10.1006/mcne.2000.0948 CrossRefPubMedGoogle Scholar
  6. 6.
    Xu XM, Chen A, Guenard V, Kleitman N, Bunge MB (1997) Bridging Schwann cell transplants promote axonal regeneration from both the rostral and caudal stumps of transected adult rat spinal cord. J Neurocytol 26(1):1–16CrossRefPubMedGoogle Scholar
  7. 7.
    Xu XM, Guenard V, Kleitman N, Bunge MB (1995) Axonal regeneration into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord. J Comp Neurol 351(1):145–160.  https://doi.org/10.1002/cne.903510113 CrossRefPubMedGoogle Scholar
  8. 8.
    Guenard V, Kleitman N, Morrissey TK, Bunge RP, Aebischer P (1992) Syngeneic Schwann cells derived from adult nerves seeded in semipermeable guidance channels enhance peripheral nerve regeneration. J Neurosci 12(9):3310–3320PubMedGoogle Scholar
  9. 9.
    Kromer LF, Cornbrooks CJ (1985) Transplants of Schwann cell cultures promote axonal regeneration in the adult mammalian brain. Proc Natl Acad Sci U S A 82(18):6330–6334CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Chirila TV, Constable IJ, Crawford GJ, Vijayasekaran S, Thompson DE, Chen YC, Fletcher WA, Griffin BJ (1993) Poly(2-hydroxyethyl methacrylate) sponges as implant materials: in vivo and in vitro evaluation of cellular invasion. Biomaterials 14(1):26–38CrossRefPubMedGoogle Scholar
  11. 11.
    Plant GW, Chirila TV, Harvey AR (1998) Implantation of collagen IV/poly(2-hydroxyethyl methacrylate) hydrogels containing Schwann cells into the lesioned rat optic tract. Cell Transplant 7(4):381–391CrossRefPubMedGoogle Scholar
  12. 12.
    Plant GW, Harvey AR, Chirila TV (1995) Axonal growth within poly (2-hydroxyethyl methacrylate) sponges infiltrated with Schwann cells and implanted into the lesioned rat optic tract. Brain Res 671(1):119–130CrossRefPubMedGoogle Scholar
  13. 13.
    Joosten EA, Bar PR, Gispen WH (1995) Collagen implants and cortico-spinal axonal growth after mid-thoracic spinal cord lesion in the adult rat. J Neurosci Res 41(4):481–490.  https://doi.org/10.1002/jnr.490410407 CrossRefPubMedGoogle Scholar
  14. 14.
    Plant GW, Christensen CL, Oudega M, Bunge MB (2003) Delayed transplantation of olfactory ensheathing glia promotes sparing/regeneration of supraspinal axons in the contused adult rat spinal cord. J Neurotrauma 20(1):1–16.  https://doi.org/10.1089/08977150360517146 CrossRefPubMedGoogle Scholar
  15. 15.
    Barbour HR, Plant CD, Harvey AR, Plant GW (2013) Tissue sparing, behavioral recovery, supraspinal axonal sparing/regeneration following sub-acute glial transplantation in a model of spinal cord contusion. BMC Neurosci 14:106.  https://doi.org/10.1186/1471-2202-14-106 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of Neurosurgery, School of MedicineStanford UniversityStanfordUSA

Personalised recommendations