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Schwann Cells pp 385-400 | Cite as

Transmission Electron Microscopy for Zebrafish Larvae and Adult Lateral Line Nerve

  • Rebecca L. Cunningham
  • Kelly R. MonkEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1739)

Abstract

Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be imaged by TEM. Here, we detail the multiday processing of larval trunks and dissected posterior lateral line for TEM, as well as how to trim embedded samples, section, and stain grids for imaging.

Key words

Transmission electron microscopy Myelin ultrastructure Lateral line nerve Motor nerves Adult zebrafish lateral line nerve 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Vollum InstituteOregon Health & Science UniversityPortlandUSA
  2. 2.Department of Developmental BiologyWashington University in St. LouisSt. LouisUSA

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