Injury to the peripheral nervous system triggers a series of well-defined events within both neurons and the Schwann cells to allow efficient axonal regeneration, remyelination, and functional repair. The study of these events has previously been done using sections of nerve material to analyze axonal regrowth, cell migration, and immune cell infiltration following injury. This approach, however, has the obvious disadvantage that it is not possible to follow, for instance, the path of regenerating axons in three dimensions within the nerve trunk or the nerve bridge. In order to provide a fuller picture of such events, we have developed a whole mount staining procedure to visualize blood vessel regeneration, Schwann cell migration, axonal regrowth, and remyelination in models of nerve injury.
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
This work was supported by a Wellcome Trust grant (WT088228) and Medical Research Council grant (MR/J012785/1) to D.B.P. We are grateful to Prof. Wendy Macklin (University of Colorado Denver School of Medicine, USA) for the gift of the PLP-EGFP transgenic mouse line.
Klymkowsky MW, Hanken J (1991) Whole-mount staining of Xenopus and other vertebrates. Methods Cell Biol 36:419–441CrossRefPubMedGoogle Scholar