Abstract
Glial cells regulate a wide variety of neuronal functions during physiological and pathological conditions. Therefore, the study of glial cells and their association with axons is of paramount importance in order to understand the physiology of the nervous system. This chapter describes a detailed protocol to prepare and stain teased nerve fibers from peripheral nerves using fluorescent indirect immunolabeling and staining with vital dyes. For immunofluorescence analysis, we describe techniques to study the axonal compartment and the expression of cytoplasmic and plasma membrane proteins in Schwann cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Court FA, Sherman DL, Pratt T et al (2004) Restricted growth of Schwann cells lacking Cajal bands slows conduction in myelinated nerves. Nature 431:191–195. https://doi.org/10.1038/nature02841
Yoshimura T, Rasband MN (2014) Axon initial segments: diverse and dynamic neuronal compartments. Curr Opin Neurobiol 27:96–102. https://doi.org/10.1016/j.conb.2014.03.004
Zhang A, Desmazieres A, Zonta B et al (2015) Neurofascin 140 is an embryonic neuronal neurofascin isoform that promotes the assembly of the node of Ranvier. J Neurosci 35:2246–2254. https://doi.org/10.1523/JNEUROSCI.3552-14.2015
Wu LMN, Williams A, Delaney A et al (2012) Increasing internodal distance in myelinated nerves accelerates nerve conduction to a flat maximum. Curr Biol 22:1957–1961. https://doi.org/10.1016/j.cub.2012.08.025
Calixto A, Jara JS, Court FA (2012) Diapause formation and downregulation of insulin-like signaling via DAF-16/FOXO delays axonal degeneration and neuronal loss. PLoS Genet 8:e1003141–e1003115. https://doi.org/10.1371/journal.pgen.1003141
Court FA, Hewitt JE, Davies K et al (2009) A laminin-2, dystroglycan, utrophin axis is required for compartmentalization and elongation of myelin segments. J Neurosci 29:3908–3919. https://doi.org/10.1523/JNEUROSCI.5672-08.2009
Court FA, Zambroni D, Pavoni E et al (2011) MMP2-9 cleavage of dystroglycan alters the size and molecular composition of Schwann cell domains. J Neurosci 31:12208–12217. https://doi.org/10.1523/JNEUROSCI.0141-11.2011
Lopez-Verrilli MA, Picou F, Court FA (2013) Schwann cell-derived exosomes enhance axonal regeneration in the peripheral nervous system. Glia 61:1795–1806. https://doi.org/10.1002/glia.22558
Oñate M, Catenaccio A, MartÃnez G et al (2016) Activation of the unfolded protein response promotes axonal regeneration after peripheral nerve injury. Sci Rep 6:21709. https://doi.org/10.1038/srep21709
Mohammadi R, Sanaei N, Ahsan S et al (2015) Stromal vascular fraction combined with silicone rubber chamber improves sciatic nerve regeneration in diabetes. Chin J Traumatol 18:212–218
Abbasipour-Dalivand S, Mohammadi R, Mohammadi V (2015) Effects of local administration of platelet rich plasma on functional recovery after bridging sciatic nerve defect using silicone rubber chamber; an experimental study. Bull Emerg Trauma 3:1–7
Acknowledgments
We wish to thank all the members of the Court Lab for their contributions to this protocol. This work was supported by the Center for Integrative Biology, Universidad Mayor, FONDECYT-1150766, Geroscience Center for Brain Health and Metabolism (FONDAP-15150012), Ring Initiative ACT1109, and Canada-Israel Health Research initiative, jointly funded by the Canadian Institutes of Health Research; the Israel Science Foundation; the International Development Research Centre, Canada; and the Azrieli Foundation, Canada.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Catenaccio, A., Court, F.A. (2018). Teased Fiber Preparation of Myelinated Nerve Fibers from Peripheral Nerves for Vital Dye Staining and Immunofluorescence Analysis. In: Monje, P., Kim, H. (eds) Schwann Cells. Methods in Molecular Biology, vol 1739. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7649-2_21
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7649-2_21
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7648-5
Online ISBN: 978-1-4939-7649-2
eBook Packages: Springer Protocols