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Schwann Cells pp 317-325 | Cite as

3D Cancer Migration Assay with Schwann Cells

  • Laura Fangmann
  • Steffen Teller
  • Pavel Stupakov
  • Helmut Friess
  • Güralp O. Ceyhan
  • Ihsan Ekin DemirEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1739)

Abstract

In pancreatic cancer, neural invasion is one of the most common paths of cancer dissemination. Classically, cancer cells actively invade nerves and cause local recurrence and pain. Three-dimensional (3D) neural migration assay has become a standard tool for scientists to study neural invasion by confronting the involved cell types. This protocol introduces Schwann cells, i.e., the most prevalent cell type in peripheral nerves, in a novel heterotypic, glia-cancer-neuron, 3D migration assay for assessing their relevance in the early pathogenesis of neural invasion. Particularly, this assay allows the monitoring of the early Schwann cell migratory activity.

Key words

3D migration assay Schwann cells Neural invasion Pancreatic cancer Dorsal root ganglia/DRG Schwann cell carcinotropism 

References

  1. 1.
    Ceyhan GO, Demir IE, Altintas B (2008) Neural invasion in pancreatic cancer: a mutual tropism between neurons and cancer cells. Biochem Biophys Res Commun 374:442–447CrossRefPubMedGoogle Scholar
  2. 2.
    Demir IE, Boldis A, Pfitzinger PL (2014) Investigation of Schwann cells at neoplastic cell sites before the onset of cancer invasion. J Natl Cancer Inst 106:dju184CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Laura Fangmann
    • 1
  • Steffen Teller
    • 1
  • Pavel Stupakov
    • 1
  • Helmut Friess
    • 1
  • Güralp O. Ceyhan
    • 1
  • Ihsan Ekin Demir
    • 1
    Email author
  1. 1.Department of Surgery, Klinikum rechts der IsarTechnische Universität MünchenMunichGermany

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