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Schwann Cells pp 149-160 | Cite as

Human Induced Pluripotent Stem Cell-Derived Sensory Neurons for Fate Commitment of Bone Marrow Stromal Cell-Derived Schwann Cells

  • Sa Cai
  • Daisy K. Y. ShumEmail author
  • Ying-Shing ChanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1739)

Abstract

Here we describe the in vitro derivation of sensory neurons for use in effecting fate commitment of Schwann cell-like cells derived from human bone marrow stromal cells (hBMSCs). We adopt a novel combination of small molecules in an 8-day program that induces the differentiation of human induced pluripotent stem cells into sensory neurons. In co-cultures, the derived sensory neurons present contact-dependent cues to direct hBMSC-derived Schwann cell-like cells toward the Schwann cell fate. These derived human Schwann cells survive passaging and cryopreservation, retain marker expression despite withdrawal of glia-inducing medium and neuronal cues, demonstrate capacity for myelination, and therefore promise application in autologous transplantation and re-myelination therapy.

Key words

Human BMSC-derived Schwann cells Induced pluripotent stem cells Small-molecule inhibitors Sensory neurons Fate commitment 

Notes

Acknowledgments

This work was supported in part by the HKRGC-General Research Fund 777810, NSFC/RGC-Joint Research Scheme N_HKU741/11, Innovation and Technology Fund (100/10), SK Yee Medical Research Fund, National Natural Science Foundation of China (81000011, 81272080), and the Strategic Research Theme on Neuroscience of The University of Hong Kong.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.School of Biomedical Sciences, Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina

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