Quality Control and Validation Issues in LC-MS Metabolomics

  • Olga Begou
  • Helen G. Gika
  • Georgios A. Theodoridis
  • Ian D. Wilson
Part of the Methods in Molecular Biology book series (MIMB, volume 1738)


Global metabolic profiling (untargeted metabolomics) of different and complex biological matrices aims to implement an holistic, hypothesis-free analysis of (potentially) all the metabolites present in the analyzed sample. However, such an approach, although it has been the focus of great interest over the past few years, still faces many limitations and challenges, particularly with regard to the validation and the quality of the obtained results. The present protocol describes a quality control (QC) procedure for monitoring the precision of the analytical process involving untargeted metabolic phenotyping of urine and plasma/serum. The described/suggested methodology can be applied to different biological matrices, such as biological biofluids, cell, and tissue extracts.

Key words

Quality control Untargeted metabolomics Biological samples 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Olga Begou
    • 1
  • Helen G. Gika
    • 2
  • Georgios A. Theodoridis
    • 1
  • Ian D. Wilson
    • 3
  1. 1.Department of ChemistryAristotle UniversityThessalonikiGreece
  2. 2.School of MedicineAristotle UniversityThessalonikiGreece
  3. 3.Department of Surgery and CancerImperial College LondonLondonUK

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