Abstract
The emergence of drug resistance threatens to destroy tuberculosis control programs worldwide, with resistance to all first-line drugs and most second-line drugs detected. Drug tolerance (or phenotypic drug resistance) is also likely to be clinically relevant over the 6-month long standard treatment for drug-sensitive tuberculosis. Transcriptional profiling the response of Mycobacterium tuberculosis to antimicrobial drugs offers a novel interpretation of drug efficacy and mycobacterial drug-susceptibility that likely varies in dynamic microenvironments, such as the lung. This chapter describes the noninvasive sampling of tuberculous sputa and techniques for mRNA profiling M. tb bacilli during patient therapy to characterize real-world drug actions.
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Acknowledgments
L.M.W. was funded by a Brazilian government agency CAPES (Coordination for the Improvement of Higher Education Personnel) PhD visiting fellowship [99999.005648/2014-09]. K.A.G. acknowledges funding from the Wellcome Trust for the Bacterial Microarray Group at St. George’s [062511, 080039, and 086547]. S.J.W. was supported by the Wellcome Trust [204538/Z/16/Z] and the PreDiCT-TB consortium (http://www.predict-tb.eu) which is funded from the Innovative Medicines Initiative Joint Undertaking under grant agreement No 115337, resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013) and EFPIA companies’ in-kind contribution.
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Wildner, L.M., Gould, K.A., Waddell, S.J. (2018). Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa. In: Gillespie, S. (eds) Antibiotic Resistance Protocols. Methods in Molecular Biology, vol 1736. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7638-6_11
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DOI: https://doi.org/10.1007/978-1-4939-7638-6_11
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