Lab-on-a-Chip Device for Rapid Measurement of Vitamin D Levels

  • Harald PeterEmail author
  • Nikitas Bistolas
  • Soeren Schumacher
  • Cecilia Laurisch
  • Paul C. Guest
  • Ulrich Höller
  • Frank F. Bier
Part of the Methods in Molecular Biology book series (MIMB, volume 1735)


Lab-on-a-chip assays allow rapid analysis of one or more molecular analytes on an automated user-friendly platform. Here we describe a fully automated assay and readout for measurement of vitamin D levels in less than 15 min using the Fraunhofer in vitro diagnostics platform. Vitamin D (25-hydroxyvitamin D3 [25(OH)D3]) dilution series in buffer were successfully tested down to 2 ng/mL. This could be applied in the future as an inexpensive point-of-care analysis for patients suffering from a variety of conditions marked by vitamin D deficiencies.

Key words

Nutritional programming Nutrient deficiency Metabolic disease Vitamins Vitamin D Lab-on-a-chip 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Harald Peter
    • 1
    Email author
  • Nikitas Bistolas
    • 1
  • Soeren Schumacher
    • 1
  • Cecilia Laurisch
    • 1
  • Paul C. Guest
    • 2
  • Ulrich Höller
    • 3
  • Frank F. Bier
    • 1
  1. 1.Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB)PotsdamGermany
  2. 2.Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of BiologyUniversity of Campinas (UNICAMP)CampinasBrazil
  3. 3.Analytical Research Center, DSM Nutritional ProductsKaiseraugstSwitzerland

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