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Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins

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Book cover Host-Pathogen Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1734))

Abstract

Constant advancements in methodology and mass spectrometry instrumentation, genome sequencing and bioinformatic tools have enabled the identification of numerous pathogen proteomes. Identifying the pathogen interacting proteins by means of high-throughput techniques is key for understanding pathogen invasion and survival mechanisms and in such a way proposing specific proteins as pharmaceutical targets. Herein we describe the methodology for the enrichment and identification of pathogen surface proteome using cell surface protein biotinylation followed by LC-MS/MS and bioinformatic analyses of such data. This strategy is to be employed for the determination of protein subcellular localization and prediction of potential pathogen interacting proteins.

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Acknowledgments

The authors acknowledge the European Commission for funding the VetMedZg ERA chair team (ERA Chair Initiative). We also acknowledge Croatian Science Foundation (project 3421) for supporting FM, HRZZ (project 4135) for supporting VM; and APVV-14-218, VEGA1/0258/15, and VEGA 1/0261/15 for supporting MB.

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Authors and Affiliations

  1. ERA Chair VetMedZg Project, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

    Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Vladimir Mrljak & Mangesh Bhide

  2. Department for Parasitology and Parasitic Diseases with Clinics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

    Franjo Martinković & Iva Štimac

  3. Laboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia

    Mangesh Bhide

  4. Institute of Neuroimmunology, Slovakia Academy of Sciences, Bratislava, Slovakia

    Mangesh Bhide

Authors
  1. Anita Horvatić
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  2. Josipa Kuleš
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  3. Nicolas Guillemin
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  4. Franjo Martinković
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  5. Iva Štimac
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  6. Vladimir Mrljak
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  7. Mangesh Bhide
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Corresponding author

Correspondence to Anita Horvatić .

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Editors and Affiliations

  1. Departamento de Biología Molecular e Ingeniería Bioquímica, Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de, Olavide/Junta de Andalucía, Seville, Spain

    Carlos Medina

  2. Department of Microbiology, University of Seville, Sevilla, Sevilla, Spain

    Francisco Javier López-Baena

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Horvatić, A. et al. (2018). Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins. In: Medina, C., López-Baena, F. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 1734. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7604-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7604-1_9

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7603-4

  • Online ISBN: 978-1-4939-7604-1

  • eBook Packages: Springer Protocols

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