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Host-Pathogen Interactions pp 71–81Cite as

Organoids as a Model to Study Infectious Disease

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1734))

Abstract

The advent of the gastric organoid culture system has provided a new model to emulate native epithelial tissue in vitro. Gastric organoids grow from isolated epithelial stem cells and develop into three dimensional structures that can be used to study host physiology. Here we describe current laboratory protocols for growing gastric organoids and the microinjection of pathogens such as Helicobacter pylori into the lumen of gastric organoids in order to study the cellular response following infection.

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Acknowledgments

This work was supported by National Institutes of Health Grants RO1 DK102551 (MHM). This project was supported in part by PHS Grant P30 DK078392 (Live Microscopy Core at University of Cincinnati) of the Digestive Disease Research Core Center in Cincinnati.

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Authors and Affiliations

  1. Department of Pharmacology and Systems Physiology, University of Cincinnati, Cincinnati, OH, USA

    Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose & Eitaro Aihara

Authors
  1. Kristen A. Engevik
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  2. Andrea L. Matthis
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  3. Marshall H. Montrose
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  4. Eitaro Aihara
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Corresponding author

Correspondence to Eitaro Aihara .

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Editors and Affiliations

  1. Departamento de Biología Molecular e Ingeniería Bioquímica, Centro Andaluz de Biología del Desarrollo, CSIC/Universidad Pablo de, Olavide/Junta de Andalucía, Seville, Spain

    Carlos Medina

  2. Department of Microbiology, University of Seville, Sevilla, Sevilla, Spain

    Francisco Javier López-Baena

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Engevik, K.A., Matthis, A.L., Montrose, M.H., Aihara, E. (2018). Organoids as a Model to Study Infectious Disease. In: Medina, C., López-Baena, F. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 1734. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7604-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7604-1_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7603-4

  • Online ISBN: 978-1-4939-7604-1

  • eBook Packages: Springer Protocols

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