Neonatal Rat Cardiomyocytes Isolation, Culture, and Determination of MicroRNAs’ Effects in Proliferation
Cardiomyocytes loss is a major contributor for many cardiovascular diseases including heart failure and myocardial infarction. Although extremely limited, adult cardiomyocytes are able to proliferate. Understanding the molecular mechanisms controlling cardiomyocytes proliferation is extremely important for enhancing cardiomyocyte proliferation to promote cardiac regeneration and repair. MicroRNAs (miRNAs, miRs) are powerful controllers of many essential biological processes including cell proliferation. Here, we described in detail a protocol for isolation and culture of neonatal rat cardiomyocytes and the determination of miRNAs’ effects in proliferation based on two well-established methods including EdU and Ki67 immunofluorescent stainings.
Key wordsCardiomyocyte MicroRNA Proliferation Ki67 EdU
This work was supported by the grants from National Natural Science Foundation of China (81570362, 91639101, and 81200169 to J.J. Xiao and 81400647 to Y. Bei), Innovation Program of Shanghai Municipal Education Commission (2017-01-07-00-09-E00042), the grant from Science and Technology Commission of Shanghai Municipality (17010500100), and the development fund for Shanghai talents (to J.J. Xiao).
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