Screening miRNA for Functional Significance by 3D Cell Culture System

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1733)

Abstract

Cell-based assays play important roles in cell biology and drug discovery. 3D cell culture, which allows cells to grow or interact with their surrounding in all three dimensions, provides more physiological information for the in vivo tests. Here, we describe a tunable collagen-based 3D cell culture system based on collagen material crosslinked with transgluminase, to study the function of miR. Methods including gel handling, proliferation assays, gene, and protein expressions in a 3D setting are described.

Key words

3D culture Col-Tgel Gelatin Transglutaminase miRNA Proliferation assay Gene expression Immunohistochemical staining 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Department of Surgery, Keck School of MedicineUniversity of Southern CaliforniaLos AngelesUSA

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