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Cancer Susceptibility Models in Protease-Deficient Mice

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Book cover Proteases and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1731))

Abstract

For decades, proteases have been associated with cancer progression due to the ability of some members of this large group of enzymes to degrade tumor cell surroundings, thereby facilitating cancer invasion and dissemination. However, the generation of mouse models deficient in proteases has revealed the existence of a great variety of functions among proteolytic enzymes in cancer biology, including important tumor-suppressive roles. Therefore, in this chapter, we describe methods to chemically induce different types of cancer (lung adenocarcinoma, hepatocellular carcinoma, oral and esophageal carcinoma, colorectal carcinoma, skin cancer, and fibrosarcoma) in genetically modified mouse models to efficiently evaluate the specific pro- or antitumoral function of proteases in cancer.

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Acknowledgments

We thank Dr. C. López-Otín for helpful comments and advice. Our work is supported by grants from Ministerio de Economía y Competitividad, Instituto de Salud Carlos III, CIBERONC, Plan Feder, and Progeria Research Foundation. S.F.-R. is recipient of an FPU Fellowship.

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Authors and Affiliations

  1. Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Oviedo, Spain

    Alicia R. Folgueras, Sandra Freitas-Rodríguez, Yaiza Español & Gloria Velasco

Authors
  1. Alicia R. Folgueras
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  2. Sandra Freitas-Rodríguez
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  3. Yaiza Español
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  4. Gloria Velasco
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Corresponding authors

Correspondence to Alicia R. Folgueras or Gloria Velasco .

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Editors and Affiliations

  1. Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Asturias, Spain

    Santiago Cal

  2. Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Asturias, Spain

    Alvaro J. Obaya

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Folgueras, A.R., Freitas-Rodríguez, S., Español, Y., Velasco, G. (2018). Cancer Susceptibility Models in Protease-Deficient Mice. In: Cal, S., Obaya, A. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 1731. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7595-2_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7595-2_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7594-5

  • Online ISBN: 978-1-4939-7595-2

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