The relevance of tumor vasculature has been extensively recognized, and it is still the focus of numerous lines of research for basic, translational, and clinical scientists. Indeed, the knowledge of some of its regulatory mechanisms has provoked the generation of ongoing cancer therapies. Within the context of the tumor microenvironment, the information that the analysis of the vasculature provides is very valuable, and it might reveal not just its quality and the response against a specific therapy but also its close relationship with neighboring stromal and tumor players.
Studies during last decades already supported the contribution of extracellular proteases in neovascularization events, including ADAMTS. However, deeper analyses are still required to better understand the modulation of their proteolytic activity in the tumor microenvironment. Future studies will clearly benefit from existing and ongoing genetically modified mouse models.
Here we emphasize the use of syngeneic models to study the vasculature during tumor progression, supported by their intact immunocompetent capacities and also by the range of possibilities to play with engineered mice and with modified tumor cells. Although various high-tech and sophisticated approaches have already been reported to evaluate tumor neovascularization, here we describe a simple and easily reproduced methodology based in the immunofluorescence detection of vascular-specific molecules. A final in silico analysis guarantees an unbiased quantification of tumor vasculature under different conditions.
Extracellular microenvironment In silico analysis Metalloproteinase Tumor stroma Vasculature
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
Work in the author’s laboratory has been supported by grants from the Ministerio de Economía y Competitividad and Instituto de Salud Carlos III from Spain and cofinanced by FEDER (PI13/00168 and PI16/00345 to JCRM). SRG is supported by a contract from Garantía Juvenil (PEJ-2014-A-38416-MINECO-FSE).
For data shown, all mice were kept in the Centro de Investigaciones Biomédicas-UGR Animal Facility under pathogen-free conditions and according to institutional guidelines.
Martino-Echarri E, Fernández-Rodríguez R, Rodríguez-Baena FJ et al (2013) Contribution of ADAMTS1 as a tumor suppressor gene in human breast carcinoma. Linking its tumor inhibitory properties to its proteolytic activity on nidogen-1 and nidogen-2. Int J Cancer 133(10):2315–2324. https://doi.org/10.1002/ijc.28271CrossRefPubMedGoogle Scholar