Advertisement

Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics

  • Lennart J. van Winden
  • Olaf van Tellingen
  • Huub H. van RossumEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

In clinical diagnostics, samples containing low testosterone cannot be analyzed by random access immunoassays normally available at clinical laboratories. For these samples, sensitive and specific LC-MS/MS-based testosterone methods are required. An LC-MS/MS-based testosterone assay is described that was developed and validated for routine clinical application.

Key words

Testosterone LC-MS/MS Steroids Hormone 

References

  1. 1.
    Marshall SK, Bangert WJ (2008) Clinical chemistry, 6th edn. Elsevier Science, EdinburghGoogle Scholar
  2. 2.
    Wang HW, Gay Y, Botelho GD, Caudill JC, Vesper SP (2014) Total testosterone quantitative measurement in serum by LC-MS/MS. Clin Chim Acta 8(5):583–592Google Scholar
  3. 3.
    van Rossum HH, Bergman AM, Lentjes E (2015) Analytical challenges and potential applications of sex steroid hormone analysis in breast and prostate cancer patients. Chromatographia 78(5–6):359–365CrossRefGoogle Scholar
  4. 4.
    Handelsman DJ, Wartofsky L (2013) Requirement for mass spectrometry sex steroid assays in the journal of clinical endocrinology and metabolism. J Clin Endocrinol Metab 98(10):3971–3973CrossRefPubMedGoogle Scholar
  5. 5.
    Hepburn S et al (2016) Sex steroid hormone stability in serum tubes with and without separator gels. Clin Chem Lab Med 54(9):1451–1459PubMedGoogle Scholar
  6. 6.
    Shi RZ, van Rossum HH, Bowen RAR (2012) Serum testosterone quantitation by liquid chromatography-tandem mass spectrometry: interference from blood collection tubes. Clin Biochem 45(18):1706–1709CrossRefPubMedGoogle Scholar
  7. 7.
    Methlie P et al (2013) Multisteroid LC-MS/MS assay for glucocorticoids and androgens, and its application in Addison’s disease. Endocr Connect 2(12):125–136CrossRefPubMedCentralGoogle Scholar
  8. 8.
    Bui HN et al (2010) Serum testosterone levels measured by isotope dilution-liquid chromatography-tandem mass spectrometry in postmenopausal women versus those in women who underwent bilateral oophorectomy. Ann Clin Biochem 47:248–252CrossRefPubMedGoogle Scholar
  9. 9.
    Kushnir MM et al (2010) Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals. Clin Chem 56(7):1138–1147CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Lennart J. van Winden
    • 1
  • Olaf van Tellingen
    • 1
  • Huub H. van Rossum
    • 1
    Email author
  1. 1.Laboratory of Clinical Chemistry and HematologyThe Netherlands Cancer InstituteAmsterdamThe Netherlands

Personalised recommendations