Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics

  • Lennart J. van Winden
  • Olaf van Tellingen
  • Huub H. van RossumEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)


In clinical diagnostics, samples containing low testosterone cannot be analyzed by random access immunoassays normally available at clinical laboratories. For these samples, sensitive and specific LC-MS/MS-based testosterone methods are required. An LC-MS/MS-based testosterone assay is described that was developed and validated for routine clinical application.

Key words

Testosterone LC-MS/MS Steroids Hormone 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Lennart J. van Winden
    • 1
  • Olaf van Tellingen
    • 1
  • Huub H. van Rossum
    • 1
    Email author
  1. 1.Laboratory of Clinical Chemistry and HematologyThe Netherlands Cancer InstituteAmsterdamThe Netherlands

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