Skip to main content

Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics

  • Protocol
Clinical Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1730))

Abstract

In clinical diagnostics, samples containing low testosterone cannot be analyzed by random access immunoassays normally available at clinical laboratories. For these samples, sensitive and specific LC-MS/MS-based testosterone methods are required. An LC-MS/MS-based testosterone assay is described that was developed and validated for routine clinical application.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 129.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Marshall SK, Bangert WJ (2008) Clinical chemistry, 6th edn. Elsevier Science, Edinburgh

    Google Scholar 

  2. Wang HW, Gay Y, Botelho GD, Caudill JC, Vesper SP (2014) Total testosterone quantitative measurement in serum by LC-MS/MS. Clin Chim Acta 8(5):583–592

    Google Scholar 

  3. van Rossum HH, Bergman AM, Lentjes E (2015) Analytical challenges and potential applications of sex steroid hormone analysis in breast and prostate cancer patients. Chromatographia 78(5–6):359–365

    Article  CAS  Google Scholar 

  4. Handelsman DJ, Wartofsky L (2013) Requirement for mass spectrometry sex steroid assays in the journal of clinical endocrinology and metabolism. J Clin Endocrinol Metab 98(10):3971–3973

    Article  CAS  PubMed  Google Scholar 

  5. Hepburn S et al (2016) Sex steroid hormone stability in serum tubes with and without separator gels. Clin Chem Lab Med 54(9):1451–1459

    PubMed  CAS  Google Scholar 

  6. Shi RZ, van Rossum HH, Bowen RAR (2012) Serum testosterone quantitation by liquid chromatography-tandem mass spectrometry: interference from blood collection tubes. Clin Biochem 45(18):1706–1709

    Article  CAS  PubMed  Google Scholar 

  7. Methlie P et al (2013) Multisteroid LC-MS/MS assay for glucocorticoids and androgens, and its application in Addison’s disease. Endocr Connect 2(12):125–136

    Article  CAS  PubMed Central  Google Scholar 

  8. Bui HN et al (2010) Serum testosterone levels measured by isotope dilution-liquid chromatography-tandem mass spectrometry in postmenopausal women versus those in women who underwent bilateral oophorectomy. Ann Clin Biochem 47:248–252

    Article  CAS  PubMed  Google Scholar 

  9. Kushnir MM et al (2010) Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals. Clin Chem 56(7):1138–1147

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Huub H. van Rossum .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

van Winden, L.J., van Tellingen, O., van Rossum, H.H. (2018). Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7592-1_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7591-4

  • Online ISBN: 978-1-4939-7592-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics