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Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis

  • Jesmond Dalli
  • Romain A. Colas
  • Mary E. Walker
  • Charles N. Serhan
Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Abstract

Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation.

Key words

Lipid mediator metabololipidomics Flux analysis Eicosanoids Resolvin Protectin Maresin Profiling Liquid chromatography-tandem mass spectrometry 

Notes

Acknowledgments

This work was supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (Grant number: 677542), a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant number: 107613/Z/15/Z), and the Barts Charity (Grant number: MGU0343). CN Serhan is supported by the National Institutes of Health, USA Grant Number P01GM095467.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Jesmond Dalli
    • 1
  • Romain A. Colas
    • 1
  • Mary E. Walker
    • 1
  • Charles N. Serhan
    • 2
  1. 1.Lipid Mediator Unit, Biochemical Pharmacology, William Harvey Research Institute, Barts and the London School of MedicineQueen Mary University of LondonLondonUK
  2. 2.Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain MedicineBrigham and Women’s Hospital and Harvard Medical SchoolBostonUSA

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