Mass Spectrometry Imaging of Metabolites
Mass spectrometry imaging (MSI) is a technique which is gaining increasing interest in biomedical research due to its capacity to visualize molecules in tissues. First applied to the field of clinical proteomics, its potential for metabolite imaging in biomedical studies is now being recognized. Here we describe how to set up experiments for mass spectrometry imaging of metabolites in clinical tissues and how to tackle most of the obstacles in the subsequent analysis of the data.
Key wordsMass spectrometry imaging Tissue metabolomics Biomarker discovery Data analysis
This work has been made possible with the support of the Dutch Province of Limburg. BB thanks the European Union (ERA-NET: TRANSCAN 2), ITEA, and RVO (ITEA 151003/ITEA 14001) for their financial support.
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